His-Tag Purification

Showing all 6 results

Product info

Catalog Id
Size
Price
h-311-25
25 mL
338.00
h-311-100
100 mL
892.00
Description

Low Density Copper Agarose Resin is available in bulk or in prepacked columns. Purification of proteins is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and different chemistry, which need to be separated.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Cu2+. The resulting resin (ready-to-use) is ideal for rapid purification of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Storage/Handling: Store at 4°C. Do NOT freeze.

Product info

Catalog Id
Size
Price
h-300-25
25 mL
338.00
h-300-100
100 mL
892.00
Description

Purification of proteins is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and different chemistry, which need to be separated.

Affinity Chromatography is the purification technique that is widely used. It is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin needs to be loaded with a divalent metal of your choosing (Ni2+, Cu2+, Zn2+ or Co2+) or can be used as a negative control to detect non-specific binding. The resulting resin (ready-to-use) is ideal for rapid purifications of His-tagged proteins.

Storage/Handling: Store at 4°C. Do NOT freeze.

Product info

Catalog Id
Size
Price
h-301-25
25 mL
338.00
h-301-100
100 mL
892.00
Description

Purification of proteins is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and different chemistry, which need to be separated.

Affinity Chromatography is the purification technique that is widely used. It is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin needs to be loaded with a divalent metal of your choosing (Ni2+, Cu2+, Zn2+ or Co2+) or can be used as a negative control to detect non-specific binding. The resulting resin (ready-to-use) is ideal for rapid purifications of His-tagged proteins.

Storage/Handling: Store at 4°C. Do NOT freeze.

Product info

Catalog Id
Size
Price
h-321-25
25 mL
338.00
h-321-100
100 mL
892.00
Description

Low Density Nickel Agarose Resin is available in bulk or in prepacked columns. It has a binding capacity of >12 mg/ml with approximately 60 kDa protein. It works very well with the His-Tag Buffer Set.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Storage/Handling: Store at 4°C. Do NOT freeze.

Note: Some discoloration of the Nickel IDA resin has been observed in reaction with low levels of reducing agents such as DTT. However, the resin remains fully functional even at concentrations as high as 5mM DTT.

Product info

Catalog Id
Size
Price
h-330-25
25 mL
338.00
h-330-100
100 mL
892.00
Description

High Density Zinc Agarose Resin is available as bulk resins for better customization. Purification of proteins is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and different chemistry, which need to be separated.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Zn2+. The resulting resin (ready-to-use) is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Storage/Handling: Store at 4°C. Do NOT freeze.

Product info

Catalog Id
Size
Price
h-331-25
25 mL
338.00
h-331-100
100 mL
892.00
Description

Low Density Zinc Agarose Resin is available as bulk resins for better customization. Purification of proteins is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and different chemistry, which need to be separated.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Zn2+. The resulting resin (ready-to-use) is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Storage/Handling: Store at 4°C. Do NOT freeze.