Metal Free

Showing all 2 results

Product info

Catalog Id
Size
Price
h-300-25
25 mL
338.00
h-300-100
100 mL
892.00
Description

Purification of proteins is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and different chemistry, which need to be separated.

Affinity Chromatography is the purification technique that is widely used. It is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin needs to be loaded with a divalent metal of your choosing (Ni2+, Cu2+, Zn2+ or Co2+) or can be used as a negative control to detect non-specific binding. The resulting resin (ready-to-use) is ideal for rapid purifications of His-tagged proteins.

Storage/Handling: Store at 4°C. Do NOT freeze.

Product info

Catalog Id
Size
Price
h-301-25
25 mL
338.00
h-301-100
100 mL
892.00
Description

Purification of proteins is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and different chemistry, which need to be separated.

Affinity Chromatography is the purification technique that is widely used. It is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin needs to be loaded with a divalent metal of your choosing (Ni2+, Cu2+, Zn2+ or Co2+) or can be used as a negative control to detect non-specific binding. The resulting resin (ready-to-use) is ideal for rapid purifications of His-tagged proteins.

Storage/Handling: Store at 4°C. Do NOT freeze.