Immobilzed Enzyme Resins

Showing all 5 results

Product info

Catalog Id
Size
Price
i-010-5
5 mL
Description

Ficin is a cysteine protease enzyme which is derived from the latex of fig trees with activity similar to other cysteine protease enzymes including endopeptidases, aminopeptidases, and dipeptidyl peptidases. Ficin cleaves mouse immunoglobulin G molecules (IgG1) in the hinge region. The concentration of cysteine changes how ficin cleaves IgG1. A F(ab’)2 fragment is formed in the presence of 1mM cysteine. The F(ab’)2 fragments are smaller than whole IgG molecules and better penetrate tissue for antigen recognition while avoiding unspecific binding to Fc receptors. In the presence of 10mM cysteine, two 2 Fab fragments are produced instead. F(ab) fragments block background from primary antibody binding, and block endogenous and exposed immunoglobulins and tissues in labeling experiments.

Immobilized Ficin Agarose Resin offers a convenient solution to the production of F(ab’)2 and Fab fragments while eliminating autolysis and protease contamination and the need to remove the enzymes following digestion. Fab and F(ab’)2 can be further separated from whole IgG with either Protein A Agarose Resin (GoldBio Product # P-400) or ion exchange chromatography.

Activity:1-1.5 mg ficin/ml of resin

Support: 6% Cross-linked Agarose

Storage/Handling: Shipped at ambient temperature. Upon receipt, store at 4°C, do NOT freeze.

Product info

Catalog Id
Size
Price
i-020-5
5 mL
304.00
i-020-25
25 mL
1,442.00
Description

Papain is a cysteine protease enzyme from papaya latex that has a wide variety of activities including endopeptidases, aminopeptidases, dipeptidyl peptidases and enzymes with both exo- and endo-peptidase activity. In particular, papain cleaves immunoglobulin G (IgG) molecules near the hinge region of the antibody, resulting in three similar, ~50 kDa, fragments; an Fc domain and two monovalent Fab domains. This papain-digested antibody is therefore unable to promote agglutination, precipitation, opsonization or lysis. The Fc fragment can then be used to determine the specificity of the Fc receptors without any typical antigen binging interference while the Fab fragments can be used in immunohistochemical studies.

Immobilized Papain Agarose Resin is a convenient reagent for the production of Fab and Fc fragments which eliminates autolysis and protease contamination, allows for better control of the digestion and eliminates the need to remove the papain enzyme after digestion. The Fab fragments can further be separated from the Fc fragment with either our Protein A Agarose Resin (GoldBio Catalog # P-400) or via ion exchange chromatography.

Immobilized Papain Agarose Resin is supplied as a 50% slurry in 50% glycerol, 0.1M sodium acetate, pH 4.4 with sodium azide as a preservative.

Activity: ≥15‐40 BAEE units/ml resin (One unit will hydrolyze 1.0 µmole of BAEE per minute at pH 6.2 at 25°C).

Capacity: 250 µg papain/ml resin

Resin: 6% Cross‐linked Agarose

Shipping/Handling: Shipped at ambient temperature. Upon receipt, store at 4°C. Do NOT freeze.

Product info

Catalog Id
Size
Price
i-030-5
5 mL
Description

Pepsin, one of the principal digestion enzymes, breaks down proteins in to smaller peptides. It is most efficient in cleaving the peptide bonds between hydrophobic and aromatic amino acids such as phenylalanine, tryptophan and tyrosine. Pepsin readily cleaves immunoglobulin G (IgG) molecules, generating F(ab’)2 fragments and small Fc peptide fragments. The smaller Fc fragments can be easily removed via gel filtration while the F(ab’)2 fragments can then be purified from any remaining, undigested IgG. The F(ab’)2 fragments are highly useful for research interests, being able to penetrate tissue better than standard IgG antibodies and facilitate better antigen recognition while avoiding unspecific binding to Fc receptors.

Immobilized Pepsin Agarose Resin is an efficient method for producing F(ab’)2 fragments from IgG molecules for antibody mimetic. Undigested IgG can be quickly removed with our Protein A Agarose Resin (GoldBio Catalog # P-400). Fc fragments can also be removed using a gel filtration system.

Resin: 6% Cross-linked Agarose

Shipping/Handling: Shipped at ambient temperature. Upon receipt, store at 4°C, do NOT freeze.

Product info

Catalog Id
Size
Price
i-040-5
5 mL
Description

Pepstatin A is a competitive, reversible aspartic protease inhibitor which inhibits proteases such as pepsin, chymosin, renin, HIV proteases, and cathepsins D and E. Peptstatin A is highly selective and is often used in a mixture with other enzyme inhibitors. In vitro studies have shown pepstatin A is the only aspartic acid inhibitor that exhibits inhibitory effects on HIV proteases.

GoldBio’s Immobilized Pepstatin Agarose Resin can be used for the purification of cathepsins, pepsin, bacterial aspartic proteases, HIV proteases and other molecules that bind pepstatin. The Pepstatin is immobilized on 6% agarose resin that is covalently coupled to pepstatin through a diaminodipropylamine (DADPA) activated spacer arm and has a binding capacity of 1-2 mg pepsin per millimeter of settled resin.

Pepstatin Agarose is supplied as a 50% slurry in 0.5M NaCl and 0.02% thimerosal.

Activity: 1-2mg pepsin/ml of resin

Resin: 6% Beaded Agarose

Shipping/Handling: Shipped at ambient temperature. Upon receipt, store at 4°C, do NOT freeze.

Product info

Catalog Id
Size
Price
i-050-2
2 mL
Description

Trypsin is a serine protease that hydrolyses proteins which can be found in the digestive system. Trypsin cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues with very little or no cleavage at lysyl-proline and arginyl-proline bonds. This special cleaving activity yields peptides that are easily identified by mass spectrometry. Additionally, our Immobilized Trypsin is TPCK treated to deactivate interfering chymotrypsin, which can interfere with sequence analysis.

Immobilized Trypsin Agarose Resin is trypsin immobilized on agarose, allowing for the cleaving of peptide bonds without the trypsin contamination in your final products.This allows for the cleaving of peptide bonds of cysteine, arginine and lysine residues and yield fragments identifiable in mass spectrometry.

Immobilized Trypsin is supplied as a 50% slurry containing glycerol and sodium azide as a preservative.

Source: Bovine

Activity: ≥200 TAME units/ml resin (1 unit = 1µmole TAME (p-toluenesulfonyl-L-arginine methyl ester) hydrolyzed/minute at pH 8.2, 25°C)

Support: 4% Cross-linked Agarose

Storage/Handling: Shipped at ambient temperature. Upon receipt store at 4°C, do NOT freeze.