High Purity Antibiotics
Showing all 5 results
Ampho99 is the purest form of amphotericin B available and should be used for the most sensitive applications. In general, GoldBio’s USP Grade amphotericin B will not adversely affect your cells and is suitable for most experiments.
Amphotericin B is a polyene antifungal isolated from Streptomyces species. While it has been suggested that Amphotericin B interacts with ergosterol of the fungal cell membrane leading to an altered cell membrane permeability and death, more recent studies show that the mechanism behind amphotericin B’s fungicidal activity is more complex. It is most effective against fungi and yeast and is often used against opportunistic fungal infections and leishmaniasis. Additionally, amphotericin B can be used to prevent growth of F. oxysporum in chickpea and P. triticina in wheat. It is soluble in acidic and basic conditions.
MW: 924.08 g/mol
Storage/Handling: Store at 2-8°C. Soluble in DMSO or DMF. This product is light sensitive.
PubChem Chemical ID: 5280965
Bacitrac99 is the most pure form of bacitracin available, and should be used for the most sensitive applications and on the most sensitive cell lines since any impurities that might cause inhibition of resistant cell lines have been removed. In general, our USP Grade bacitracin will not adversely affect your cells and is suitable for most experiments.
Bacitracin is a cyclic polypeptide antibiotic isolated from Bacillus subtilis. It is made up of a variety of antimicrobial peptide fractions including A, A1, B, B1, B2, C, D, E, F, G, and X. This standard grade product is a mixture of these fractions, with bacitracin A exhibiting the most potent antimicrobial activity. Bacitracins B1 and B2 exhibit about 90% of the antimicrobial activity of bacitracin A when together. Other forms of bacitracin have not been studied extensively, but may have negligible antimicrobial properties.
Bacitracin targets the cell wall of gram-positive organisms such as Streptococcus pyogenes and Staphylococcus aureus. Bacitracin also functions as a useful tool to distinguish between group A and group B β-hemolytic Streptococcus species, which are susceptible and resistant, respectively.
Bacitracin functions through inhibiting phosphorylation of bactoprenol, a step essential to synthesize peptidoglycan. Consequently, cell lysis occurs. Bacitracin resistance is conferred through a BcrABC transporter that pumps bacitracin out of cells or through BacA which allows phosphorylation of bactoprenol via an alternative biosynthesis pathway.
Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.
MW: 1,422.69 g/mol
Storage/Handling: Store at 4°C.
PubChem Chemical ID: 24891503
Cyclohex99 is the most pure form of cycloheximide available. It is over 98% pure and all toxic isomers have been removed. Cyclohex99 should be used for the most sensitive applications as it is free of impurities. In general, GoldBio’s Molecular Grade cycloheximide is suitable for most experiments.
Cycloheximide is a protein synthesis inhibitor in eukaryotic cells. It prevents ribosomal mRNA translocation during protein translation. Cycloheximide can be used to study cellular processes and inhibit yeast growth. It has also been used to determine half-life of a protein using western blotting techniques of protein cell lysate. Additionally, it has been used to select for CHX-resistant strains of yeast and fungi. It also displays neuroprotective properties.
MW: 281.35 g/mol
Storage/Handling: Store at room temperature.
PubChem Chemical ID: 6167
Air shipments may require special packaging and a small additional box charge.
Hygro99 is the purest form of Hygromycin B available. It is over 99% pure and should be used for the most sensitive applications and on the most sensitive cell lines. All impurities that might cause inhibition of resistant cell lines have been removed. In general, our Molecular Grade Hygromycin B (which is still ≥92 % pure and less expensive) will not adversely affect your cells and is suitable for most experiments.
Both Hygro99 and Molecular Grade Hygromycin B appear equal in the concentration of their functional units per mg of material as measured in our GoldBio hygromycin unit assay. However, this apparent anomaly is most likely due to the level of sensitivity of the hygromycin assay.
Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for selection of hygromycin resistance gene transformed cells.
This product has been tested against both sensitive and resistant cells at Gold Biotechnology Labs.
ONLY AVAILABLE IN CONTINENTAL UNITED STATES
Hygromycin B, 99% Pure
Formula: C 20H37N3O13
MW: 563.52 g/mol
Storage/Handling: Store desiccated at -20°C.
PubChem Chemical ID: 35766