Buffers

Showing 1–16 of 22 results

Product info

Catalog Id
Size
Price
i-905-50
50 mL
176.00
i-905-100
100 mL
252.00
Description

This Buffer Set consists of:
– 5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl)
– 25 mL 3M Recrystallized Imidazole Solution, pH 6.0

This Buffer set allows researchers to develop, wash and elute buffers for His-tag protein purification.

Product info

Catalog Id
Size
Price
p-500-50
50 mL
81.00
p-500-100
100 mL
114.00
p-500-250
250 mL
162.00
Description

5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl).

Phosphate Buffer, pH8 is a mix of Sodium Phosphate dibasic (Na2HPO4) and Monosodium Phosphate (NaH2PO4). This buffer is the basis for wash and elution buffers for protein purification through His-tag.

Product info

Catalog Id
Size
Price
a-010-25
25 g
38.00
a-010-50
50 g
58.00
a-010-100
100 g
75.00
Description

ACES is a zwitterionic buffering agent used in biochemistry and molecular biology that was first selected and described by Good et al. It is an acetamido buffer that is useful for a pH range of 6.1 – 7.5. ACES is used as buffering component in cell culture media, in both agarose and polyacrylamide electrophoresis, and in isoelectric focusing of proteins. ACES forms a complex with most common metals and formation constants should be taken into account when using this buffer in a solution containing metal ions. ACES absorbs UV light at 230 nm.

Product Specifications
ACES
N-(Carbamoylmethyl)-2-Aminoethane Sulfonic Acid

HIGH PURITY GRADE

Formula: C4H10N2O4S

MW: 182.20 g/mol

Storage/Handling: Store at room temperature.

PubChem Chemical ID: 81832

ADA

Product info

Catalog Id
Size
Price
a-780-25
25 g
28.00
a-780-50
50 g
36.00
a-780-100
100 g
52.00
Description

ADA is a zwitterionic buffering agent used in biochemistry and molecular biology that was first selected and described by Good et al. It is an acetamido buffer that is useful for a pH range of 6.0 – 7.2. ADA forms a complex with most common metals and stability constants should be taken into account when using this buffer. It has been used as a complexing agent to effectively help remove contaminant metals from soil. ADA absorbs UV light between 0.1 and 260 nm. Solubility of ADA increases if the free acid is converted to the sodium salt with NaOH.

Product Specifications
ADA;
MHA;
N-(2-acetamido)iminodiacetic acid;
N-(carbamoylmethyl)iminodiacetic acid

ULTRA PURE GRADE

Formula: C6H10N2O5

MW: 190.22 g/mol

PubChem Chemical ID: 117765

Storage/Handling: Store at room temperature.

Product info

Catalog Id
Size
Price
gb-177-100
100 mL
81.00
gb-177-500
500 mL
159.00
gb-176-100
100 Preps
240.00
gb-176-250
250 Preps
422.00
gb-176-500
500 Preps
777.00
Description

The Bacterial Cell Lysis Kit has been developed for the extraction of biologically active, soluble proteins and inclusion bodies from bacterial cells. The Bacterial Lysis Buffer is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The kit’s lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Bacterial Cell Lysis Buffer is based on organic buffering agents and utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as reducing agents chelating agent, and protease inhibitors may be added into Bacterial Cell Lysis Buffer. This reagent has been tested for use with several widely used bacteria including E. coli strains. Bacterial Cell Lysis Buffer eliminates the need for laborious mechanical lysis of bacterial cells and removal of DNA/RNA with nuclease treatments. The proprietary combination of this reagent provides a simple and versatile method of bacterial protein extraction and isolation of inclusion bodies.

The Bacterial Cell Lysis Kit (GB-176; 100, 250 or 500 preps) includes a separate tube of modified lysozyme preparation that contains nucleases and results in optimal lysis and minimal contamination. This kit contains all of the reagents needed to perform protein extraction.  For extracting soluble proteins, one prep is suitable for extracting approximately 300 mg wet cell pellets for every 1 ml Bacterial Cell Lysis Buffer. When the kits are used for extracting soluble proteins from spheroplasts, one prep is suitable for extracting approximately 90 mg wet cell pellets for every 40 μl Bacterial Suspension Buffer. Bacterial Lysis Buffer is also available separately for further downstream applications.

BES

Product info

Catalog Id
Size
Price
b-780-25
25 g
28.00
b-780-50
50 g
44.00
b-780-100
100 g
60.00
Description

BES is a zwitterionic buffering agent used in biochemistry and molecular biology that was first selected and described by Good et al. It is a bis(2-hydroxyethyl)amine buffer that is useful for a pH range of 6.4 – 7.8. It is soluble in water at 0°C up to 3.2M. Like most neutral, amine-based buffers, BES forms a complex with DNA. It interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This lack of interference occurs because steric hindrance of the protonated tertiary amine in BES shields the negative charge of DNA to a lesser extent than less substituted buffers. BES can be used in a buffered saline solution in the calcium phosphate mediated transfection of eukaryotic cells with plasmid DNA. BES also forms a complex with certain metals, including copper and cobalt. Though BES binds to coppers ions, it is still suitable for use with the bicinchoninic acid (BCA) assay. Diminished protein-induced color development can be accounted for or even eliminated by precipitating protein prior to reaction with bicinchoninic acid.

Product Specifications
BES;
N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic Acid;
N,N-Bis(2-hydroxyethyl)taurine

ULTRA PURE GRADE

Formula: C6H15NO5S

MW: 213.25 g/mol

PubChem Chemical ID: 73243

Storage/Handling: Store at room temperature.

Product info

Catalog Id
Size
Price
c-870-25
25 g
30.00
c-870-50
50 g
36.00
c-870-100
100 g
52.00
c-870-500
500 g
195.00
Description

CHES is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a cyclohexylamine buffer that is useful for a pH range of 8.6 – 10.0 and commonly used to study enzymatic processes above physiological pH. CHES displays only weak or negligible complexation capabilities for some metals making it a suitable non-coordinating buffer for use in most solutions containing metal ions. CHES displays high affinity for the iodoacetate binding site of liver alcohol dehydrogenase, protecting the enzyme from inactivation by iodoacetic acid.

Product Specifications
CHES;
2-(Cyclohexylamino)ethanesulfonic acid;
N-Cyclohexyltaurine;

ULTRA PURITY GRADE

Formula: C8H17NO3S

MW: 207.29 g/mol

PubChem Chemical ID: 66898

Storage/Handling: Store at room temperature.

Product info

Catalog Id
Size
Price
h-400-100
100 g
48.00
h-400-250
250 g
96.00
h-400-500
500 g
146.00
h-400-1
1 kg
226.00
h-400-2
2 kg
378.00
h-400-5
5 kg
802.00
Description

HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay.

HEPES free acid can be mixed with HEPES sodium salt to achieve the desired pH. Alternatively, HEPES free acid can be titrated with NaOH or KOH to achieve the desired pH.

Product Specifications
HEPES
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid

ULTRA PURE GRADE

Formula: C8H18N2O4S

MW: 238.30 g/mol

Storage/Handling: Store at room temperature.

PubChem Chemical ID: 23831

Product info

Catalog Id
Size
Price
h-401-50
50 g
46.00
h-401-100
100 g
78.00
h-401-500
500 g
231.00
h-401-1
1 kg
387.00
h-401-2-5
2.5 kg
1,265.00
h-401-5
5 kg
1,490.00
Description

HEPES sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay.

HEPES sodium salt can be mixed with HEPES free acid to achieve the desired pH.

Product Specifications
HEPES, Sodium Salt
sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate

HIGH PURITY GRADE

Formula: C8H17N2O4SNa

MW: 260.29 g/mol

Storage/Handling: Store in a dry and well-ventilated place.

PubChem Chemical ID: 2724248

Product info

Catalog Id
Size
Price
h-480-25
25 g
58.00
h-480-50
50 g
81.00
h-480-100
100 g
112.00
Description

HEPPSO is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 7.1 – 8.5. It is commonly used as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPPSO displays no toxicity for crustaceans with which it has been studied and has been deemed suitable for toxicological studies. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. HEPPSO forms a complex with Cu(II) ions so stability constants and concentrations should be taken into consideration when using this buffer in a solution containing copper ions. Though HEPPSO binds to coppers ions, it is still suitable for use with the bicinchoninic acid (BCA) assay. Diminished protein-induced color development can be accounted for or even eliminated by precipitating protein prior to reaction with bicinchoninic acid.

Product Specifications
HEPPSO;
N-(Hydroxyethyl)piperazine-N’-2-hydroxypropanesulfonic acid;
N-Cyclohexyltaurine;

ULTRA PURE GRADE

Formula: C9H20N2O5S

MW: 268.33 g/mol

PubChem Chemical ID: 100205

Storage/Handling: Store at room temperature.

Product info

Catalog Id
Size
Price
i-906-250
1 kit
Description

His-Tag Buffer Set includes:
– 250 mL Wash Buffer (50mM Phosphate Buffer pH 8, 300mM NaCl, 10mM Imidazole)
– 50 mL Elution Buffer (50mM Phosphate Buffer, 300mM NaCl, 500mM Imidazole)

Designed for use with Goldbio Nickel and Cobalt Agarose resins, but it can be used with most standard His-tag purification resins.

Product info

Catalog Id
Size
Price
gb-180-100
100 mL
81.00
gb-180-500
500 mL
165.00
Description

Mammalian Cell Lysis Buffer has been developed for extraction of total soluble proteins from mammalian cultured cells. The Mammalian Cell Lysis Buffer is based on organic buffering agents, which utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Mammalian Cell Lysis Buffer. Mammalian Cell Lysis Buffer reagent has been tested for use with a wide variety of mammalian cells. Mammalian Cell Lysis Buffer can be used for both suspension as well as adherent cells. The proprietary combination of this reagent provides a simple and versatile method for the extraction of proteins from mammalian cells.


Product Specifications
Mammalian Cell Lysis Buffer

Storage/Handling: Store at 4°C.

Product info

Catalog Id
Size
Price
m-090-100
100 g
76.00
m-090-250
250 g
117.00
m-090-500
500 g
171.00
m-090-1
1 kg
292.00
Description

MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography. MES is often employed as a substitute for the highly toxic cacodylate, as well as a substitute for the non-zwitterionic buffers citrate and malate. MES complexes with some organotin(IV) molecules that are being explored as antitumor agents.

MES free acid or MES free acid monohydrate can be mixed with MES sodium salt to acquire desired pH. Alternatively, MES free acid or MES free acid monohydrate can be titrated with sodium hydroxide to attain desired pH.

Product Specifications
MES Free Acid, Monohydrate, Ultra Pure
2-(N-Morpholino)ethanesulfonic acid, Monohydrate

Formula: C6H13NO4S ∙ H2O

MW: 213.25 g/mol

Storage/Handling: Store in a dry and well-ventilated place.

PubChem Chemical ID: 16218417

Note: MES Hydrate (CAS# 1266615-59-1) and MES Monohydrate (CAS# 145224-94-8) differ only in the number of water molecules associated with the buffer in powder form. Regardless of the number of water molecules present, these buffers are identical when dissolved into an aqueous solution.

Product info

Catalog Id
Size
Price
m-095-25
25 g
28.00
m-095-50
50 g
36.00
m-095-100
100 g
52.00
m-095-250
250 g
100.00
m-095-1
1 kg
344.00
Description

MES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography. MES is often employed as a substitute for the highly toxic cacodylate, as well as a substitute for the non-zwitterionic buffers citrate and malate. MES complexes with some organotin(IV) molecules that are being explored as antitumor agents.

MES free acid or MES free acid monohydrate can be mixed with MES sodium salt to acquire desired pH. Alternatively, MES free acid or MES free acid monohydrate can be titrated with sodium hydroxide to attain desired pH.

Product Specifications
MES;
2-morpholinoethanesulfonic acid;
2-(N-morpholino)ethanesulfonic acid;

ULTRA PURE GRADE

Formula: C6H13NO4S

MW: 195.24 g/mol

PubChem Chemical ID: 78165

Storage/Handling: Store at room temperature.

Product info

Catalog Id
Size
Price
m-091-25
25 g
24.00
m-091-50
50 g
39.00
m-091-100
100 g
62.00
m-091-500
500 g
153.00
m-091-1
1 kg
246.00
Description

MES sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography. MES is often employed as a substitute for the highly toxic cacodylate, as well as a substitute for the non-zwitterionic buffers citrate and malate. MES complexes with some organotin(IV) molecules that are being explored as antitumor agents.

MES sodium salt can be mixed with MES free acid or MES free acid monohydrate to attain desired pH.

Product Specifications
MES Sodium Salt
sodium;2-morpholin-4-ylethanesulfonate;
Sodium;2-(N-Morpholino)ethanesulfonic acid

Reagent Grade

Formula: C6H12NO4SNa

MW: 217.22 g/mol

Storage/Handling: Store in a dry and well-ventilated place.

PubChem Chemical ID: 23673676

Product info

Catalog Id
Size
Price
m-795-25
25 g
28.00
m-795-50
50 g
44.00
m-795-100
100 g
60.00
Description

MOPSO is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer, structurally similar to MOPS, that is useful for a pH range of 6.2 – 7.6. It is commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification with chromatography. Due to low ionic mobility, MOPSO is regarded as an excellent buffer for use in capillary electrochromatography. MOPSO lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It does form a complex with some organotin(IV) molecules that are being explored as antitumor agents. MOPSO also interacts with the peptide backbone of bovine serum albumin (BSA) to stabilize BSA against thermal denaturation.

MOPSO free acid can be mixed with MOPSO sodium salt to acquire desired pH. Alternatively, MOPSO free acid can be titrated with sodium hydroxide to attain desired pH.

Product Specifications
MOPSO;
β-Hydroxy-4-morpholinepropanesulfonic acid;
3-Morpholino-2-hydroxypropanesulfonic acid;

ULTRA PURE GRADE

Formula: C7H15NO5S

MW: 225.26 g/mol

PubChem Chemical ID: 109333

Storage/Handling: Store at room temperature.