Buffers & Washes For Chromatography

Showing all 3 results

Product info

Catalog Id
Size
Price
i-905-50
50 mL
176.00
i-905-100
100 mL
252.00
Description

This Buffer Set consists of:
– 5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl)
– 25 mL 3M Recrystallized Imidazole Solution, pH 6.0

This Buffer set allows researchers to develop, wash and elute buffers for His-tag protein purification.

Product info

Catalog Id
Size
Price
p-500-50
50 mL
81.00
p-500-100
100 mL
114.00
p-500-250
250 mL
162.00
Description

5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl).

Phosphate Buffer, pH8 is a mix of Sodium Phosphate dibasic (Na2HPO4) and Monosodium Phosphate (NaH2PO4). This buffer is the basis for wash and elution buffers for protein purification through His-tag.

Product info

Catalog Id
Size
Price
i-906-250
1 kit
Description

His-Tag Buffer Set includes:
– 250 mL Wash Buffer (50mM Phosphate Buffer pH 8, 300mM NaCl, 10mM Imidazole)
– 50 mL Elution Buffer (50mM Phosphate Buffer, 300mM NaCl, 500mM Imidazole)

Designed for use with Goldbio Nickel and Cobalt Agarose resins, but it can be used with most standard His-tag purification resins.