Buffers & Washes For Chromatography
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This Buffer Set consists of:
– 5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl)
– 25 mL 3M Recrystallized Imidazole Solution, pH 6.0
This Buffer set allows researchers to develop, wash and elute buffers for His-tag protein purification.
5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl).
Phosphate Buffer, pH8 is a mix of Sodium Phosphate dibasic (Na2HPO4) and Monosodium Phosphate (NaH2PO4). This buffer is the basis for wash and elution buffers for protein purification through His-tag.
His-Tag Buffer Set includes:
– 250 mL Wash Buffer (50mM Phosphate Buffer pH 8, 300mM NaCl, 10mM Imidazole)
– 50 mL Elution Buffer (50mM Phosphate Buffer, 300mM NaCl, 500mM Imidazole)
Designed for use with Goldbio Nickel and Cobalt Agarose resins, but it can be used with most standard His-tag purification resins.