Lysis Buffers

Showing all 5 results

Product info

Catalog Id
Size
Price
gb-177-100
100 mL
87.00
gb-177-500
500 mL
170.00
gb-176-100
100 Preps
252.00
gb-176-250
250 Preps
441.00
gb-176-500
500 Preps
813.00
Description

The Bacterial Cell Lysis Kit has been developed for the extraction of biologically active, soluble proteins and inclusion bodies from bacterial cells. The Bacterial Lysis Buffer is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The kit’s lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Bacterial Cell Lysis Buffer is based on organic buffering agents and utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as reducing agents chelating agent, and protease inhibitors may be added into Bacterial Cell Lysis Buffer. This reagent has been tested for use with several widely used bacteria including E. coli strains. Bacterial Cell Lysis Buffer eliminates the need for laborious mechanical lysis of bacterial cells and removal of DNA/RNA with nuclease treatments. The proprietary combination of this reagent provides a simple and versatile method of bacterial protein extraction and isolation of inclusion bodies.

The Bacterial Cell Lysis Kit (GB-176; 100, 250 or 500 preps) includes a separate tube of modified lysozyme preparation that contains nucleases and results in optimal lysis and minimal contamination. This kit contains all of the reagents needed to perform protein extraction. For extracting soluble proteins, one prep is suitable for extracting approximately 300 mg wet cell pellets for every 1 ml Bacterial Cell Lysis Buffer. When the kits are used for extracting soluble proteins from spheroplasts, one prep is suitable for extracting approximately 90 mg wet cell pellets for every 40 μl Bacterial Suspension Buffer. Bacterial Lysis Buffer is also available separately for further downstream applications.

Product info

Catalog Id
Size
Price
gb-180-100
100 mL
87.00
gb-180-500
500 mL
176.00
Description

Mammalian Cell Lysis Buffer has been developed for extraction of total soluble proteins from mammalian cultured cells. The Mammalian Cell Lysis Buffer is based on organic buffering agents, which utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Mammalian Cell Lysis Buffer. Mammalian Cell Lysis Buffer reagent has been tested for use with a wide variety of mammalian cells. Mammalian Cell Lysis Buffer can be used for both suspension as well as adherent cells. The proprietary combination of this reagent provides a simple and versatile method for the extraction of proteins from mammalian cells.


Product Specifications
Mammalian Cell Lysis Buffer

Storage/Handling: Store at 4°C.

Product info

Catalog Id
Size
Price
gb-181-100
100 mL
87.00
gb-181-500
500 mL
170.00
Description

Tissue Cell Lysis Buffer has been developed for extraction of total soluble protein from fresh or frozen animal tissues. Tissue Cell Lysis Buffer is based on an organic buffer, which utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Tissue Cell Lysis Buffer. Tissue Cell Lysis Buffer reagent has been tested for use with a wide variety of animal tissues.

.

Product info

Catalog Id
Size
Price
gb-179-100
100 mL
180.00
gb-179-500
500 mL
366.00
Description

Product info

Catalog Id
Size
Price
gb-178
100 Preps
Description