IPTG

Showing all 2 results

Product info

Catalog Id
Size
Price
i2481c
1 g
24.00
i2481c5
5 g
38.00
i2481c25
25 g
162.00
i2481c50
50 g
260.00
i2481c100
100 g
494.00
i2481c200
200 g
954.00
i2481c300
300 g
1,272.00
i2481c400
400 g
1,652.00
i2481c500
500 g
1,990.00
Description

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

IPTG is commonly used in cloning procedures that require induction of β-galactosidase and is most often used with X-Gal (Gold Bio #X4281C) or Bluo-Gal (Gold Bio #B-673) for blue/white colony screening or Magenta-Gal (Gold Bio #B-378) for red/white colony screening of bacterial colonies.

IPTG is also used in the induction of recombinant proteins. In those systems, a protein of interest is encoded downstream of the IPTG inducible promoter. In the presence of IPTG, the protein of interest is induced in the cell culture. The culture can then be lysed and the protein expressed and purified through a number of methods, including His Tag or GST purification systems (for proteins with ligand tags).

GoldBio IPTG is made from a synthetic, non-animal origin.

Product Specifications:
IPTG
Isopropyl-beta-D-thiogalactoside

MOLECULAR BIOLOGY GRADE

Forumla: C9H18O5S

MW: 238.30 g/mol

Purity: >99% by HPLC

Dioxane free

Storage/Handling: Store desiccated at -20°C. Protect from light.

PubChem Chemical ID: 656894

Product info

Catalog Id
Size
Price
i2481-ez10
10 mL
81.00
i2481-ez50
50 mL
129.00
i2481-ez100
100 mL
212.00
Description

The IPTG EZ Pak™ is the fastest and easiest way to make a set amount of sterile IPTG solution. The kit includes preweighed IPTG powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio IPTG and the sterile solution is ready for tissue culture, bacterial media, or any other appropriate use.

The 50 ml and 100 ml require a vacuum manifold for easy vacuum filtering, while the 10 ml pack size comes with a syringe filter.

IPTG is commonly used with X-Gal (Gold Bio #X4281C) or Bluo-Gal (Gold Bio #B-673) for blue/white colony screening or Magenta-Gal (Gold Bio #B-378) for red/white colony screening of bacterial colonies.

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

IPTG is also used in the induction of recombinant proteins. In those systems, a protein of interest is encoded downstream of the IPTG inducible promoter. In the presence of IPTG, the protein of interest is induced in the cell culture. The culture can then be lysed and the protein expressed and purified through a number of methods, including His Tag or GST purification systems (for proteins with ligand tags).

GoldBio IPTG is made from a synthetic, non-animal origin.

PubChem Chemical ID: 656894