DNA & Protein Electrophoresis

Showing 1–16 of 62 results

Product info

Catalog Id
Size
Price
d010-500
1x500 μl
66.00
d010-1000
2x500 μl
123.00
d010-1500
3x500 μl
172.00
d010-2000
4x500 μl
228.00
d010-2500
5x500 μl
261.00
d010-3000
6x500 μl
285.00
Description

A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 13 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 250-10,000 base pairs. The 1K and 3K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples. This ladder is ready-to-use, and premixed with loading buffer dye for direct loading on gel.

Loading dye contains Bromophenol Blue as the tracking dye.

Range: 250bp – 10kb

Number of bands: 13

Recommended Load: 5 μl/well (0.5 μg)

Stable for shipping at ambient temperatures

Storage/Handling:
Store at 25°C for 6 months.
Store at -20°C for 24 months.

Product info

Catalog Id
Size
Price
d011-500
1x500 μl
82.00
d011-1000
2x500 μl
154.00
d011-1500
3x500 μl
220.00
d011-2000
4x500 μl
278.00
d011-2500
5x500 μl
326.00
d011-3000
6x500 μl
358.00
Description

The 1 kb PLUSTM is composed of 14 individual DNA fragments: 25K, 10K, 8K, 6K, 5K, 4K, 3K, 2.5K, 2K, 1500, 1000, 750, 500, 250 base pairs. This product contains two enhanced bands (3K and 1K bp) for easy reference. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

Loading dye contains orange G & xylene cyanol FF as tracking dyes.

Range: 250bp – 25kb

Number of bands: 14

Recommended Load: 5 μl/well (0.5 μg)

Stable for shipping at ambient temperatures

Storage/Handling:
Store at 25°C for 6 months.
Store at -20°C for 24 months.

Product info

Catalog Id
Size
Price
d001-500
1x500 μl
66.00
d001-1000
2x500 μl
123.00
d001-1500
3x500 μl
172.00
d001-2000
4x500 μl
228.00
d001-2500
5x500 μl
261.00
d001-3000
6x500 μl
285.00
Description

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

Loading dye contains orange G & xylene cyanol FF as tracking dyes.

Range: 100bp – 1500bp

Number of bands: 11

Recommended Load: 5 μl/well (0.5 μg)

Stable for shipping at ambient temperatures

Storage/Handling:
Store at 25°C for 6 months.
Store at -20°C for 24 months.

Product info

Catalog Id
Size
Price
d003-500
1x500 μl
66.00
d003-1000
2x500 μl
123.00
d003-1500
3x500 μl
180.00
d003-2000
4x500 μl
228.00
d003-2500
5x500 μl
261.00
d003-3000
6x500 μl
285.00
Description

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 12 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-3,000 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

Loading dye contains orange G & xylene cyanol FF as tracking dyes.

Range: 100bp – 3000bp

Number of bands: 12

Recommended Load: 5 μl/well (0.5 μg)

Stable for shipping at ambient temperatures

Storage/Handling:
Store at 25°C for 6 months.
Store at -20°C for 24 months.

Product info

Catalog Id
Size
Price
d100-500
1x500 μl
82.00
d100-1000
2x500 μl
154.00
d100-1500
3x500 μl
220.00
d100-2000
4x500 μl
278.00
d100-2500
5x500 μl
326.00
d100-3000
6x500 μl
358.00
Description

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 17 fragments, suitable for gel electrophoresis. The DNA includes fragments ranging from 50-1,500 base pairs. The 200 and 500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 g a load) for approximating the mass of DNA in comparably intense samples of a similar size. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

Loading dye contains orange G as the tracking dye.

Range: 50bp – 1500bp

Number of bands: 17

Recommended Load: 5 μl/well (0.5 μg)

Stable for shipping at ambient temperatures

Storage/Handling:
Store at 25°C for 6 months.
Store at -20°C for 24 months.

Product info

Catalog Id
Size
Price
l002-5
5 ml
38.00
l002-10
2 x 5 ml
68.00
l002-20
4 x 5 ml
128.00
l002-50
10 x 5 ml
300.00
Description

GoldBio’s 6× Blue DNA Loading Dye is pre-mixed buffer designed for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Bromophenol blue) for tracking the DNA migration. The Xylene cyanol FF and Bromophenol blue migrate at approximately 4,000 bp and 500 bp on a standard 1% TAE agarose gel respectively (800 bp and 150 bp on a 2% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Composition
0.03% Xylene cyanol FF
0.03% Bromophenol blue
10mM Tris-HCl (pH 8.0)
60% glycerol
60mM EDTA

Storage/Handling: Store at 4°C.

Product info

Catalog Id
Size
Price
g-730-1
1 ml
Description

GelRed™ is an ultra-sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed™ Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer and eliminates the need to add fluorescent DNA dye to the agarose gel during casting.

6X GelRed™ Prestain Loading Buffer with Blue Tracking Dyes contains two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel.  For smaller tracking dyes, see Catalog # G-735 (6X GelRed™ Prestain Loading Buffer with Orange Tracking Dyes).

GelRed™ and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed without changing your existing imaging system. In addition, GelRed™ is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed™ is compatible with downstream applications such as sequencing and cloning. GelRed™ is efficiently removed from DNA by commercial gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.

A series of safety tests have confirmed that GelRed™ is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed™ can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal.

Features

  • Safer than Ethidum Bromide
Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic
 
  • Easy disposal
Passed environmental safety tests for direct disposal down the drain or in regular trash

  • Ultra-sensitive
Higher sensitivity than EtBr and SYBR® Safe

  • Extremely stable
Available in water, stable at room temperature for long-term storage and microwavable
 
  • Simple to use
Very simple procedures for precast or post-electrophoresis gel staining
 
  • Compatible with a standard UV transilluminator
     
Replaces EtBr with no optical setting change
 
  • Compatible with downstream applications
Compatible with gel purification, restriction digest, sequencing and cloning
 

Storage/Handling: Store at 4°C. Protect from light.

Product may be shipped at ambient temperature without reducing performance. Please place at the recommended storage conditions upon receipt of the product.

GelRed and its uses are covered by US patent numbers 7960498, 7803943, and 8232050. SYBR is trademark of Molecular Probes, Inc.

Product info

Catalog Id
Size
Price
g-735-1
1 ml
Description

GelRed™ is an ultra-sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed™ Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer and eliminates the need to add fluorescent DNA dye to the agarose gel during casting.

6X GelRed™ Prestain Loading Buffer with Orange Tracking Dye contains an orange electrophoresis tracking dyes that run at approximately 50 bp in a 1% agarose gel. For larger tracking dyes, see Catalog # G-730 (6X GelRed™ Prestain Loading Buffer with Blu Tracking Dyes).

GelRed™ and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed without changing your existing imaging system. In addition, GelRed™ is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed™ is compatible with downstream applications such as sequencing and cloning. GelRed™ is efficiently removed from DNA by commercial gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.

A series of safety tests have confirmed that GelRed™ is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed™ can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal.

Features

  • Safer than Ethidum Bromide
Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic
 
  • Easy disposal
Passed environmental safety tests for direct disposal down the drain or in regular trash
 
  • Ultra-sensitive
Higher sensitivity than EtBr and SYBR® Safe
 
  • Extremely stable
Available in water, stable at room temperature for long-term storage and microwavable
 
  • Simple to use
Very simple procedures for precast or post-electrophoresis gel
 
  • Compatible with a standard UV transilluminator
     
Replaces EtBr with no optical setting change
 
  • Compatible with downstream applications
Compatible with gel purification, restriction digest, sequencing and cloning
 

Storage/Handling: Store at 4°C. Protect from light.

Product may be shipped at ambient temperature without reducing performance. Please place at the recommended storage conditions upon receipt of the product.

GelRed and its uses are covered by US patent numbers 7960498, 7803943, and 8232050. SYBR is trademark of Molecular Probes, Inc.

Product info

Catalog Id
Size
Price
l001-5
5 ml
38.00
l001-10
2 x 5 ml
68.00
l001-20
4 x 5 ml
128.00
l001-50
10 x 5 ml
300.00
Description

GoldBio’s 6× Green DNA Loading Dye is a pre-mixed buffer for tracking DNA samples during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Orange G) for tracking the DNA migration. The Xylene cyanol FF and Orange G migrate at approximately 4,000 bp and 50 bp on a standard 1% TAE agarose gel respectively (800 bp and 30 bp on a 2% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Composition
0.03% Xylene cyanol FF
0.15% Orange G
10mM Tris-HCl (pH 8.0)
60% glycerol
60mM EDTA

Storage/Handling: Store at 4°C.

Product info

Catalog Id
Size
Price
a-201-100
100 g
129.00
a-201-500
500 g
477.00
a-201-1000
1 kg
795.00
Description

Agarose LE (Low Electroendosmosis) is Molecular Biology Grade and is specifically designed for the superior separation of nucleic acids, with maximally crisp band resolution, and is also ideal for cloning and cloning related experiments.

GoldBio Agarose LE is refined in an advanced process that excludes the use of organic solvents. The result is a cleaner end-product with significantly reduced environmental impact. The agarose can be used for analyses of nucleic acids from 50 bp to 25 kbp, protein electrophoresis and various blotting protocols.

The low EEO of the agarose promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO reduces band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions.

Agarose is a natural product that forms an inert matrix used in electrophoresis, chromatography and other molecular biology and biochemistry techniques. Likewise, it is neutral and easily derivatizable, so it is easy to bind to its structure proteins like enzymes, antigens or antibodies. Toxicity absence makes working with agarose very convenient.

Technical Specifications
Agarose LE

Molecular Biology Grade

EEO (Electroendomosis): ≤0.12
Sulfate: ≤0.1%
Gel Strength (1%): ≥1200 g/cm 2
Gelling Temperature: 36±1.5°C
Melting Temperature: 88±1.5°C
DNase/RNase Activity: None Detected
DNA Resolution ≥1000 bp: Finely Resolved                                            
Gel Background: Very Low
DNA Binding: Very Low 

   
 

Product info

Catalog Id
Size
Price
b-785-25
25 g
26.00
b-785-50
50 g
33.00
b-785-100
100 g
45.00
b-785-500
500 g
180.00
b-785-1
1 kg
298.00
Description

Bicine is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, bis(2-hydroxyethyl)amine buffer that is useful for a pH range of 7.6 – 9.0. It is commonly used in protein crystallization, in the study of enzymatic reactions, and for electrophoresis. It is used in thin layer ion exchange chromatography for protein resolution, as well as a multiphasic buffer system for SDS-PAGE of proteins. It is soluble in water at 0°C up to 1.1 M. Bicine forms a complex with most common metals so stability constants and concentrations should be taken into consideration when choosing this buffer.

Product Specifications
Bicine;
N,N-bis(2-hydroxyethyl)glycine;
di(hydroxyethyl)glycine;
N,N- bis(hydroxyethyl)aminoacetic acid

ULTRA PURE GRADE

Formula: C6H13NO4

MW: 163.17 g/mol

PubChem Chemical ID: 8761

Storage/Handling: Store at room temperature.

Product info

Catalog Id
Size
Price
b-020-100
100 g
76.00
b-020-250
250 g
117.00
b-020-500
500 g
178.00
b-020-1
1 kg
308.00
Description

Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer that is useful for a pH range of 5.8 – 7.2. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.

Product Specifications
Bis-Tris, Ultra Pure
Bis-(2-Hydroxyethyl) Amino-Tris(Hydroxymethyl) Methane

Formula: C8H19NO5

MW: 209.24 g/mol

Storage/Handling: Keep in a dry and in a well-ventilated place.

PubChem Chemical ID: 81462

Product info

Catalog Id
Size
Price
p-810-1
1 L
Description

Blazin’ Blue™ Protein Gel Stain is a ready-to-use protein gel staining solution that is a safer and more effective replacement for traditional Coomassie stains. Blazin’ Blue™ offers a faster alternative to protein staining (5-60 minutes) in a single step without the need for fixation or washing. Protein bands can be detected through visible blue staining or by near-infrared fluorescence. And since Blazin’ Blue™ is aqueous based, there are no hazardous chemicals like methanol or acetic acid to worry about. Blazin’ Blue™ Protein Gel Stain is certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

Blazin’ Blue™ has comparable sensitivity to the standard Coomassie Blue stains, with a detection limit around 10-20 ng, depending on the detection method used (staining intensity varies between proteins). Blazin’ Blue™ staining is also fully compatible with mass spectrometry (MS) and Edman-based sequencing.

Storage/Handing: Store at 4°C.
Product may be shipped at ambient temperature without reducing performance. Please place at the recommended storage conditions upon receipt of the product.

Product info

Catalog Id
Size
Price
p-820-1
1 L
Description

Blazin’ Bright™ Luminescent Protein Gel Stain is a ready-to-use luminescent protein gel staining solution that is a safer and more effective replacement for traditional fluorescent staining. Blazin’ Bright™ offers a faster alternative to protein staining (5-60 minutes) in a single step without the need for fixation (destaining is optional). The Blazin’ Bright™ gel stains are aqueous based, there are no hazardous chemicals like methanol or acetic acid to worry about. Blazin’ Bright™ Protein Gel Stain is certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

Blazin’ Bright™ has a detection limit around 1-10 ng, depending on the detection method used (staining intensity varies between proteins) and does not cause gel shrinkage. Blazin’ Bright™ staining is also fully compatible with mass spectrometry (MS) and Edman-based sequencing.

Also check out our Blazin’ Bright™ Luminescent UV Protein Gel Stain which is optimized for use with a UV transilluminator (not compatible with blue light illuminators or laser-based scanners).

Storage/Handing: Store at room temperature.

Product info

Catalog Id
Size
Price
p-825-1
1 L
Description

Blazin’ Bright™ Luminescent UV Protein Gel Stain is a ready-to-use luminescent protein gel staining solution that is a safer and more effective replacement for traditional fluorescent staining. Gels stained with our Blazin’ Bright™ Luminescent UV Protein Gel Stain can be visualized rapidly (5-30 minutes) in a single step without fixation (destaining is optional). The Blazin’ Bright™ gel stains are aqueous based, there are no hazardous chemicals like methanol or acetic acid to worry about. Blazin’ Bright™ Protein Gel Stain is certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

Blazin’ Bright™ Luminescent UV has a detection limit around 1-10 ng, depending on the detection method used (staining intensity varies between proteins) and does not cause gel shrinkage. Blazin’ Bright™ staining is also fully compatible with mass spectrometry (MS) and Edman-based sequencing.

Also check out our Blazin’ Bright™ Luminescent Protein Gel Stain, which can be imaged using a UV transilluminator, visible light gel imager, or laser-based gel scanner (such as a Typhoon® scanner). Blazin’ Bright™ Luminescent UV gives a better signal on a UV box than Blazin’ Bright™ Luminescent, but is not compatible with a blue light illuminator or laser-based scanner.

Storage/Handing: Store at room temperature.

Product info

Catalog Id
Size
Price
p008-500
1x500 μl
164.00
p008-1000
2x500 μl
318.00
p008-1500
3x500 μl
454.00
p008-2000
4x500 μl
591.00
p008-2500
5x500 μl
717.00
p008-3000
6x500 μl
837.00
Description

• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel,
  respectively.
• 1.5~2.5 μl per well for general Western transferring.
• Apply more for thicker (>1.5 mm) or larger gel.

The BLUEstainTM 2 Protein ladder is a three-color protein standard with 13 prestained proteins, covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).

The BLUEstainTM 2 Protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute or add reducing agent before loading.

Storage/Handling: Store at 4°C for 3 months. Store at -20°C for 24 months.
This product is stable for shipping at ambient temperatures.

Contents: Approximately 0.1~0.4 mg/ml of each protein in the bufffer (20mM Tris-phosphate, pH 7.5 at 25°C), 2% SDS, 10mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol).