Coomassie Stains

Showing all 3 results

Product info

Catalog Id
Size
Price
p-810-1
1 L
Description

Blazin’ Blue™ Protein Gel Stain is a ready-to-use protein gel staining solution that is a safer and more effective replacement for traditional Coomassie stains. Blazin’ Blue™ offers a faster alternative to protein staining (5-60 minutes) in a single step without the need for fixation or washing. Protein bands can be detected through visible blue staining or by near-infrared fluorescence. And since Blazin’ Blue™ is aqueous based, there are no hazardous chemicals like methanol or acetic acid to worry about. Blazin’ Blue™ Protein Gel Stain is certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

Blazin’ Blue™ has comparable sensitivity to the standard Coomassie Blue stains, with a detection limit around 10-20 ng, depending on the detection method used (staining intensity varies between proteins). Blazin’ Blue™ staining is also fully compatible with mass spectrometry (MS) and Edman-based sequencing.

Storage/Handing: Store at 4°C.
Product may be shipped at ambient temperature without reducing performance. Please place at the recommended storage conditions upon receipt of the product.

Product info

Catalog Id
Size
Price
c-460-5
5 g
36.00
c-460-25
25 g
50.00
c-460-50
50 g
94.00
Description

Coomassie Brilliant Blue G-250 (CBBG) is a member of the Coomassie family of triphenylmethane dyes and is used as a common analytical dye in SDS-PAGE and BN-PAGE. CBBG can also be used with the Bradford Method to determine protein concentration. G-250 is differentiated from the R-250 Coomassie stain by the addition of two methyl groups and the slightly greenish tint to its blue color. CBBG may exist in one of three forms: a cation (red form) at a pH below 0 with an absorbance peak at 470 nm, an anion (blue form) at a pH above 2 with an absorbance peak at 595 nm, and a neutral, green form at a pH around 1 with an absorbance peak around 650 nm. The blue, anionic form is what binds with amino acid residues, such as arginine or aromatics, to form a stable complex—forming the basis of the Bradford Method.

Recently, it has also been shown that CBBG is also an antagonist to P2X7 (P2X purinoceptor 7: P2RX7). CBBG is soluble in water but methanol and acetic acid are often added for better solubility.

Product Specifications
Comassie Brilliant Blue G-250, Ultra Pure
CBBG, Ultra Pure

Formula: C47H48N3O7S2Na

MW: 854.02 g/mol

Storage/Handling: Store in a dry and well-ventilated place.

PubChem Chemical ID: 61363

Product info

Catalog Id
Size
Price
c-461-5
5 g
40.00
c-461-25
25 g
58.00
c-461-100
100 g
110.00
Description

Coomassie Brilliant Blue R-250 (CBBR) is a member of the Coomassie family and is used extensively as an analytical dye in SDS-PAGE.

CBBR can also be used in the Bradford Assay to help quantify the protein concentration in a mixture, like cell lysates.

Product Specifications
Comassie Brilliant Blue R-250, Ultra Pure
CBBR

Formula: C45H44N3O7S2Na

MW: 825.97 g/mol

Storage/Handling: Store in a dry and well-ventilated place.

PubChem Chemical ID: 61365