PCR Products

Showing all 10 results

Product info

Catalog Id
Size
Price
d-900-1
1 ml
38.00
d-900-2
2x1 ml
70.00
d-900-4
4x1 ml
134.00
d-900-10
10x1 ml
315.00
Description

GoldBio’s 2’-Deoxynucleoside 5’-triphosphate mix is an aqueous solution containing dATP, dCTP, dGTP and dTTP, each at a final concentration of 10mM. This mixture has been tested for performance in a wide size-range of PCR templates with Taq and Pfu DNA polymerases.

The dNTP solution is ideal for PCR, cDNA synthesis, DNA sequencing and labeling procedures, and it has been performance tested with Q-PCR and long PCR (30 kb).

Product Specifications
dNTP Mix

Certified free of nucleases (DNase, RNase) and phosphatases

Concentration: 10mM each (dATP, dCTP, dGTP and dTTP)

Storage/Handling: Store at -20°C.
Avoid freeze/thaw cycles or exposure to frequent temperature changes.

Product info

Catalog Id
Size
Price
e-675-50
50 μl
Description

Product info

Catalog Id
Size
Price
e-670-1
1 ml Trial Size
97.00
e-670-5
5 x 1 ml
419.00
Description

Product info

Catalog Id
Size
Price
g-700-1
1 ml
87.00
g-700-5
5 x 1 ml
300.00
Description

Goof-Proof™ qPCR Master Mix is an EvaGreen dye-based hot start master mix for use in DNA melt curve analysis and in Real Time PCR (RT-PCR) applications. Goof-Proof™ is a mistake-prevention master mix which contains a low concentration of blue dye. Additionally, Goof-Proof™ includes a DNA template buffer which has a higher concentration of blue dye. With the addition of the 2X Goof-Proof™ Master Mix to the reaction, it will appear light blue (see Figure 1). And when you add the template, containing DNA template buffer, the color will turn darker blue (see Figure 2).

Goof-Proof™ allows you to see quickly and easily whether you forgot to add the master mix or the template to any of your reactions, preventing pipetting mistakes and wasted time, reagents and money. The 2X Goof-Proof™ Master Mix can also be used without the Template Buffer, if desired.

                                                       

    

 

    

Figure1: 1x Goof-Proof™
Master Mix
    Figure 2: 1x Goof-Proof™
Master Mix after adding
1X Goof-Proof™ Template
Buffer

Kit components

  • Goof-Proof™ 2X Master Mix –
  • The 2X Master Mix contains GoldBio HotStart Taq DNA Polymerase, dNTPs and a low concentration of an inert blue dye. The dye helps the user distinguish between empty wells and wells containing master mix.

  • Goof-Proof™ Template Buffer –
  • The 40X Template Buffer contains a high concentration of inert blue dye. Adding the template buffer to the reaction mix makes it “Goof-Proof” to track which wells have had template added to the PCR reactions. Template Buffer is not required for the Master Mix to function, but is designed to help alleviate mistakes with template addition.

Note: Goof-Proof™ qPCR Master Mix does not include ROX reference dye. For instruments that require either a low or high concentration of ROX reference dye, we offer Goof-Proof™ qPCR Master Mix with Rox (Catalog # G-705) which includes a separate vial of ROX Reference dye, and instructions for how much ROX to add depending on which protocol and instrument you are using.

Storage/Handling: Store the kit at -20°C.

Product may be shipped on blue ice without reducing performance. Please place at the recommended storage conditions upon receipt of the product.

Product info

Catalog Id
Size
Price
g-705-1
1 ml
87.00
g-705-5
5 x 1 ml
300.00
Description

Goof-Proof™ qPCR Master Mix is an EvaGreen dye-based hot start master mix for use in DNA melt curve analysis and in Real Time PCR (RT-PCR) applications. Goof-Proof™ is a mistake-prevention master mix which contains a low concentration of blue dye. Additionally, Goof-Proof™ includes a DNA template buffer which has a higher concentration of blue dye. With the addition of the 2X Goof-Proof™ Master Mix to the reaction, it will appear light blue (see Figure 1). And when you add the template, containing DNA template buffer, the color will turn darker blue (see Figure 2).

Goof-Proof™ allows you to see quickly and easily whether you forgot to add the master mix or the template to any of your reactions, preventing pipetting mistakes and wasted time, reagents and money. The 2X Goof-Proof™ Master Mix can also be used without the Template Buffer, if desired.

                                                       

    

 

    

Figure1: 1x Goof-Proof™
Master Mix
    Figure 2: 1x Goof-Proof™
Master Mix after adding
1X Goof-Proof™ Template
Buffer

Kit components

  • Goof-Proof™ 2X Master Mix –
    The 2X Master Mix contains GoldBio HotStart Taq DNA Polymerase, dNTPs and a low concentration of an inert blue dye. The dye helps the user distinguish between empty wells and wells containing master mix.
  • Goof-Proof™ Template Buffer –
    40X Template Buffer contains a high concentration of inert blue dye. Adding the template buffer to the reaction mix makes it “Goof-Proof” to track which wells have had template added to the PCR reactions. Template Buffer is not required for the Master Mix to function, but is designed to help alleviate mistakes with template addition.
  • ROX Reference Dye –
    Proof™ qPCR Master Mix with ROX includes a vial of ROX reference dye. ROX is necessary for accurate Ct determination from well to well with certain instruments (such as some ABI and Stratagene PCR instruments, refer to your instrument instructions to see if ROX is required). ROX may add noise to melt curve analysis, which could be mistaken for real peaks. In the case of unexpected peaks, un-check “ROX” in the “Passive Reference Dye” box in the system software to prevent data collection from the ROX fluorescence channel and re-analyze the data.

Note: We also offer Goof-Proof™ qPCR Master Mix (Catalog # G-700) without ROX for instruments that do not require the ROX reference dye.

Storage/Handling: Store the kit at -20°C.

Product may be shipped on blue ice without reducing performance. Please place at the recommended storage conditions upon receipt of the product.

Product info

Catalog Id
Size
Price
g-710-1
1 ml
94.00
g-710-5
5 x 1 ml
410.00
Description

Goof-Proof™ Universal Probe Master Mix is a high-performance 2X master mix for fluorescent probe-based PCR applications such as SNP genotyping and quantitation. Goof-Proof™ has broad instrument compatibility, can be used in both standard and fast protocols and shows a high degree of consistency in both singleplex and multiplex reactions. The Goof-Proof™ Universal Probe Master Mix is perfectly designed for all fluorescent probe technologies, including displacement probes such as Molecular Beacon and hydrolysis probes such as TaqMan®.

                                                       

    

Figure 1: Reaction after
adding template with
1X Goof-Proof™ Tracking
Buffer

Kit components

  • GoldBio HotStart Taq DNA Polymerase and dNTPs in an optimized buffer suitable for high qPCR sensitivity and multiplex reactions. Note: Primers, probe and template not included.
  • Goof-Proof™ Tracking Buffer –
    The 40X Tracking Buffer contains an inert blue dye. Adding the tracking buffer to the DNA template makes it “Goof-Proof” to track which reactions have had template added to the PCR reactions. Alternatively, Goof-Proof™ Tracking Buffer can be added to the master mix to distinguish wells with reaction buffer from empty wells. Tracking Buffer is not required for the reaction to function, but is designed to help alleviate mistakes.

Note: Goof-Proof™ Universal Probe Master Mix does not include ROX reference dye. For instruments that require either a low or high concentration of ROX reference dye, we offer Goof-Proof™ Universal Probe Master Mix with ROX (Catalog # G-715) which includes a separate vial of ROX Reference dye, and instructions for how much ROX to add depending on which protocol and instrument you are using.

Storage/Handling: Store the kit at -20°C.

Product may be shipped on blue ice without reducing performance. Please place at the recommended storage conditions upon receipt of the product.

Product info

Catalog Id
Size
Price
g-715-1
1 ml
94.00
g-715-5
5 x 1 ml
410.00
Description

Goof-Proof™ Universal Probe Master Mix with ROX is a high-performance 2X master mix for fluorescent probe-based PCR applications such as SNP genotyping and quantitation. Goof-Proof™ has broad instrument compatibility, can be used in both standard and fast protocols and shows a high degree of consistency in both singleplex and multiplex reactions. The Goof-Proof™ Universal Probe Master Mix is perfectly designed for all fluorescent probe technologies, including displacement probes such as Molecular Beacon and hydrolysis probes like TaqMan®.

                                                       

    

Figure 1: Reaction after
adding template with
1X Goof-Proof™ Tracking
Buffer

Kit components

  • GoldBio HotStart Taq DNA Polymerase and dNTPs in an optimized buffer suitable for high qPCR sensitivity and multiplex reactions. Primers, probe and template not included.
  • Goof-Proof™ Tracking Buffer –
    The 40X Tracking Buffer contains an inert blue dye. Adding the tracking buffer to the DNA template makes it “Goof-Proof” to track which reactions have had template added to the PCR reactions. Alternatively, Goof-Proof™ Tracking Buffer can be added to the master mix to distinguish wells with reaction buffer from empty wells. Tracking Buffer is not required for the reaction to function, but is designed to help alleviate mistakes.
  • ROX Reference Dye –
    ROX is necessary for accurate Ct determination from well to well with certain instruments (such as some ABI and Stratagene PCR instruments, refer to your instrument instructions to see if ROX is required). ROX may add noise to melt curve analysis, which could be mistaken for real peaks. In the case of unexpected peaks, un-check “ROX” in the “Passive Reference Dye” box in the system software to prevent data collection from the ROX fluorescence channel and re-analyze the data.

Note: We also offer Goof-Proof™ Universal Probe Master Mix (Catalog # G-710) without ROX for instruments that do not require the ROX reference dye.

Storage/Handling: Store the kit at -20°C.

Product may be shipped on blue ice without reducing performance. Please place at the recommended storage conditions upon receipt of the product.

Product info

Catalog Id
Size
Price
t-513-100
100 reactions
60.00
t-513-500
500 reactions
195.00
Description

Hot start Taq DNA Polymerase 2x master mix is ready to use premix which contains hot start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer that has been optimized for routine PCR applications. Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

Product Includes

  • Hot Start Taq 2x master mix
  • 5x GC Enhancer

Taq Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)

Taq Concentration (1x): 0.1 units/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.

Source: E. coli strain expressing a Taq DNA Polymerase gene from Thermus aquaticus YT-1

Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.

Product info

Catalog Id
Size
Price
t-512-100
100 reactions
105.00
t-512-500
500 reactions
315.00
Description

Hot start Taq DNA Polymerase 2x master mix is ready to use premix which contains hot start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer that has been optimized for routine PCR applications. Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

Product Includes

  • Hot Start Taq 2x master mix
  • 5x GC Enhancer

Taq Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)

Taq Concentration (1x): 0.1 units/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.

Source: E. coli strain expressing a Taq DNA Polymerase gene from Thermus aquaticus YT-1

Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.

Product info

Catalog Id
Size
Price
p-660-50
50 reactions
112.00
p-660-200
200 reactions
360.00
Description

Pfu DNA polymerase is a heat stable DNA polymerase which has 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. This product is supplied with an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

The 2x Premix with Dye comes ready to use, containing Pfu DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer. It has been optimized for routine PCR applications.

Product Includes

  • Pfu DNA Polymerase 2x Premix
  • 5x GC Enhancer

Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)

Pfu Concentration (1x): 0.1 unit/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.

Source: E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus

Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.