Enzymes, Inhibitors & Substrates

1-Dodecylimidazole is grouped with the imidazole organic compounds, containing an imidazole 1,3-C3N2 ring produced by natural products like alkaloids. Imidazole is amphoteric and aromatic. When an additional carbon and two hydrogens bind to the ring of this compound, 1-Dodecylimidazole can produce a 1-dodecyl-3-methylimidazolium cation.

As an antifungal, 1-Dodecylimidazole can inhibit sterol C14 demethylation at low levels, 2,3-oxidosqualene cyclization and subsequent transmethylation when the quantity is raised. This has been postulated as a result of its binding to sterol carrier proteins. It functions as a hypocholesterolaemic agent by inhibiting cholesterol biosynthesis at the level of 2,3-oxidosqualene sterol cyclase.

This substance has been an effective antimalarial agent within 15 minutes of administration to in vitro cultures of the pathogen. It exhibited inhibition of protein synthesis and the alkalinization of the parasitic food vacuole. The chemical has also been observed functioning as a lysosomotropic detergent against Saccharomyces cerevisiae.

Product Specifications
1-Dodecylimidazole
N-Dodecylimidazole
1-dodecyl-1H-imidazole

Formula: C₁₅H₂₈N₂

MW: 236.40 g/mol

Storage/Handling: Store at 4°C.

PubChem Chemical ID: 78002

1-O-Methyl-α- D- galactopyranoside
1-O-Methyl-galactoside
1-OMe-alpha-D-Gal

MOLECULAR BIOLOGY GRADE

C₇H₁₄O₆

MW: 194.19 g/mol

White to off-white crystalline powder

A gratuitous α-galactoside inducer.

Storage/Handling: Store at 4°C. Protect from light.

PubChem CID: 76935

1-O-Methyl-mannose
1-OMe-α-D-Man

MOLECULAR BIOLOGY GRADE

C₇H₁₄O₆

MW: 194.18 g/mol

1-O-Stearyl-2-O-methyl-sn-glycero-3-phosphocholine
Edelfosine

MOLECULAR BIOLOGY GRADE

C₂₇H₅₈NO₆P

MW: 523.74 g/mol

Storage/Handling: Store at -15°C, and protect from light.

2-Naphthyl-beta-D-galactopyranoside is a substrate for beta-galactosidase. 2-naphthol is released upon hydrolyzation. By simultaneous coupling with a suitable staining reagent, such as hexazonium p-rosaniline, the corresponding red-brown azo-dye is formed. Naphthols can also be detected by fluorescence analysis.

Product Specifications
2-Naphthyl-β- D-galactopyranoside
β-Naphthyl-β-galactoside;
β-Nap-β-D-Gal

MOLECULAR BIOLOGY GRADE

Formula: C₁₆H₁₈O₆

MW: 306.32 g/mol

Storage/Handling: Store at -20°C. Protect from light.

PubChem Chemical ID: 24802360

2-Naphthyl-α- L-fucopyranoside is a chromogenic substrate for α-L-Fucosidase. 2-naphthol is released upon hydrolyzation. By simultaneous coupling with a suitable staining reagent, such as hexazonium p-rosaniline, the corresponding azo-dye is formed. Naphthols can also be detected by fluorescence analysis.

Product Specifications
2-Naphthyl-α- L-fucopyranoside
β-Naphthyl-α-L-fucoside
β-Nap-α-L-Fuc

MOLECULAR BIOLOGY GRADE

Formula: C₁₆H₁₈O₅

MW: 290.32 g/mol

Storage/Handling: Store at -20°C. Protect from light.

PubChem Chemical ID: 6454626

2-Nitrophenyl-N-acetyl- β-D- glucosaminide is a chromogenic β-glucosaminidase substrate yielding a yellow solution upon cleavage.

Product Specifications
2-Nitrophenyl-N-acetyl- β-D- glucosaminide
o-Nitrophenyl-2-acetamido-2-deoxy-beta-D-glucoside
ONP-beta-D-GlcNAc

MOLECULAR BIOLOGY GRADE

Formula: C₁₄H₁₈N₂O₈

MW: 342.30 g/mol

Storage/Handling: Store at -20°C. Protect from light.

Tetrazolium red is used to visualize dehydrogenase enzyme activity. Initially, the tetrazolium solution is colorless but changes to red when it comes into contact with hydrogen. Tetrazolium red is used in a biochemical viability test for seeds. The test relies on dehydrogenase enzymes to release hydrogen ions, which subsequently reduce the colorless tetrazolium salt solution to a red compound called formazan. Living cells turn red while dead cells remain colorless.

Product Specifications
2,3,5-Triphenyltetrazolium chloride
Tetrazolium Red
Triphenyl tetrazolium chloride
TTC
Tetrazolium chloride

MOLECULAR BIOLOGY GRADE

Formula:C₁₉H₁₅N₄Cl

MW:334.81 g/mol

Color/Purity:Off-white to yellow powder
Forms colorless to yellow (practically clear) solution when dissolved 5% in ethanol or water.

PubChem Chemical ID:9283

Storage/Handling:Store at 5 °C, and protect from light.

3-Indoxyl phosphate bis (2-amino-2-methyl- 1,3-propanediol) salt
Y™-phosphate bis(2-amino-2-methyl-1,3-propanediol) salt
Y™-phos • 2 AMPD

MOLECULAR BIOLOGY GRADE

C₈H₈NO₄P·2C₄H₁₁NO₂

MW: 423.41 g/mol

White to off-white powder

Histochemical substrate for alkaline phosphatase.

Storage/Handling: Store desiccated at -20°C. Protect from light.

PubChem CID: 24802232

P-aminophenyl phosphate (pAPP) can be used as a substrate for alkaline phosphatase enzymatic reactions in order to electrochemically quantify ALP activity.

The enzymatic reaction results in the production of phenol, which can be quantified and used to analyze ALP concentrations.

ALP is a naturally occurring enzyme and can be found in human tissue in the liver, bone and intestines. Usual concentrations of ALP range between 25-100 IU/L. A significant spike outside of that range could act as a signal for many diseases.

Product Specifications
p-aminophenyl phosphate (pAPP)
4-Aminophenyl phosphate monosodium salt hydrate

MOLECULAR BIOLOGY GRADE

Solid crystalline powder

Formula: C ₆H₇NO₄PNa

MW: 211.90 g/mol

Storage/Handling: Store at -15 °C, and protect from light.

PubMed Chemical ID: 71433871

4-Aminophenyl-β-D-gal

MOLECULAR BIOLOGY GRADE

C₁₂ H₁₇ N O₆

MW: 271.27 g/mol

4-Aminophenyl-β-D-galactopyranoside is a substrate for β-galactosidase. It is also a weak β-D-galactosidase inducer and can be used in affinity chromatography for isolation of galactose-binding. The 4-aminophenyl releases upon cleavage by β-galactosidase. It can then be assayed by electro-oxidation to 4-imino quinone while recording changes in the potential or current galvanic system.

Storage/Handling: Store dry at -20°C. Protect from light.

Substrate for horseradish peroxidase (HRP). HRP catalyzes oxidation of 4-chloro-1-naphthol in the presence of peroxide to the blue, insoluble product 4-chloro-1-naphthon. It is used for both blotting and immunohistochemical staining, but is less sensitive than other precipitating HRP substrates.

Product Specifications
4-Chloro-1-naphthol
4CN

MOLECULAR BIOLOGY GRADE

Formula: C₁₀H₇ClO

MW: 178.62 g/mol

Storage/Handling: Store at -20°C. Protect from light.

PubChem ID: 11787