X-gal, Bluo-gal, & Magenta-gal

Showing all 3 results

Product info

Catalog Id
Size
Price
b-673-250
250 mg
66.00
b-673-500
500 mg
116.00
b-673-1
1 g
164.00
b-673-2-5
2.5 g
432.00
b-673-10
10 g
789.00
Description

Bluo-Gal is used as an alternative to X-Gal, and produces a darker blue color. Bluo-Gal is hydrolyzed by β-galactosidase to produce a blue precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Bacterial colonies containing active β-galactosidase produce blue colonies when grown on plates containing Bluo-Gal.

Bluo-Gal is commonly used in conjunction with IPTG (GoldBIo #I2481) for blue-white screening.

Product Specifications:
Bluo-Gal
5-Bromo-3-indolyl-β-D-galactopyranoside
Blue-Gal, Lapis™-β-D-Gal

MOLECULAR BIOLOGY GRADE

Formula: C14H16BrNO6

MW: 374.18 g/mol

Purity: >99% by HPLC

Soluble in dimethylformamide (DMF)

Storage/Handling: Store at -15°C, and protect from light.

PubChem Chemical ID: 44161406

Product info

Catalog Id
Size
Price
b-378-5
5 g
330.00
b-378-10
10 g
598.00
b-378-25
25 g
1,359.00
b-378-50
50 g
2,464.00
Description

Magenta-Gal is used as an alternative to X-Gal, and produces a red color. Magenta-Gal is hydrolyzed by β-galactosidase to produce a red precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Bacterial colonies containing active β-galactosidase produce red colonies when grown on plates containing Magenta-Gal.

Magenta-Gal is commonly used in conjunction with IPTG (GoldBIo #I2481) for red/white screening.

Product Specifications:
5-Bromo-6-chloro-3-indoxyl β-D- galactopyranoside
MagentaTM-β-D-galactoside; MagentaTM-β-D-Gal
Red-Gal

MOLECULAR BIOLOGY GRADE

Formula: C14H15BrClNO6

MW: 408.63 g/mol

Storage/Handling: Store at -15°C in a dark, dry place.

PubChem Chemical ID: 44140954

Product info

Catalog Id
Size
Price
x4281c
1 g
58.00
x4281c10
10 g
324.00
Description


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5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is most commonly known as X-Gal. X-gal is a histochemical substrate for β-galactosidase which cleaves X-Gal to yield an insoluble blue precipitate.

X-Gal is a mainstay in molecular biology cloning applications in which it is used to detect the activity of β-galactosidase. X-Gal is used to detect the insertion of foreign DNA into the lacZ region of a plasmid DNA. Insertion of DNA into the lacZ region results in the loss of β-galactosidase activity. Bacteria cells that retain active β-galactosidase will result in characteristic blue colonies. Successful disruption of the lacZα gene disrupts the α-complementation of the β-galactosidase gene and the precipitate does not form, resulting in white colonies.

Many other applications also use X-Gal as a substrate to detect β-galactosidase activity. These include β-galactosidase -antibody linked immunoassays and immunohistochemistry, coliphage detection based on β-galactosidase induction and the detection of micrometastasis formation during tumor progession.

X-gal is commonly used in conjunction with IPTG (GoldBio #I2481) for blue-white screening.

Product Specifications:
X-Gal;
5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside

MOLECULAR BIOLOGY GRADE

Formula: C14H15BrClNO6

MW: 408.63 g/mol

Purity: Greater than 99% (HPLC)

Solution: Clear (2% in DMF)

Biological Activity: Blue color with Lambda, pUC, and M13

Storage/Handling: Store at -20°C. Protect from Light.

PubChem Chemical ID: 619018