Enzymes

Showing 1–16 of 30 results

Product info

Catalog Id
Size
Price
a-440-1
1 KU
98.00
a-440-5
5 KU
390.00
Description

Alkaline phosphatase is an enzyme commonly used for the removal of terminal phosphates from the 5′ end of DNA or RNA strands to prevent reannealing of the cohesive ends and facilitate future radiolabeling of the strands.

This system includes 30 U/μL of alkaline phosphatase in a 50% glycerol solution and a dilution buffer that allows for the preparation of a 3 U/μL solution for use in DNA modification.

Product Specifications
Alkaline Phosphatase, 2-Component System

BIOTECHNOLOGY GRADE

Storage/Handling: Store at 4 °C. DO NOT FREEZE.

Product info

Catalog Id
Size
Price
c-529-50
50 µl
225.00
c-529-250
250 µl
742.00
Description

Cas9 Nuclease is the purified recombinant Streptococcus pyogenes Cas9 enzyme containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR/Cas9-mediated genome editing. The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie blue staining.

CRISPR (Clustered regularly interspaced short palindromic repeats)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. The CRISPR system consists of a short non-coding guide RNA (sgRNA) made up of a target complementary CRISPR RNA (crRNA) and an auxiliary transactivating crRNA (tracrRNA). The sgRNA guides the Cas9 endonuclease to a specific genomic locus via base pairing between the crRNA sequence and the target sequence, and cleaves the DNA to create a double-strand break. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence.

Cas9 nuclease is free from detectable RNase, Endonuclease (nicking) and non-specific DNase activities.

Product Includes

  • Cas9 Nuclease (1600 ng/µl (10µM))
  • 10x Cas9 Nuclease Reaction Buffer

Source: E. coli BL21 (DE3) strain expressing a Cas9 gene from Streptococcus pyogenes with an N-terminal 6xHis tag and C-terminal SV40 nuclear localization signal (NLS)

Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.

Product info

Catalog Id
Size
Price
c-519-250
250 µl
Description

Cas9 Nuclease is the purified recombinant Streptococcus pyogenes Cas9 enzyme containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR/Cas9-mediated genome editing. The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie blue staining.

CRISPR (Clustered regularly interspaced short palindromic repeats)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. The CRISPR system consists of a short non-coding guide RNA (sgRNA) made up of a target complementary CRISPR RNA (crRNA) and an auxiliary transactivating crRNA (tracrRNA). The sgRNA guides the Cas9 endonuclease to a specific genomic locus via base pairing between the crRNA sequence and the target sequence, and cleaves the DNA to create a double-strand break. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence.

Cas9 nuclease is free from detectable RNase, Endonuclease (nicking) and non-specific DNase activities.

Product Includes

  • Cas9 Nuclease (160 ng/µl (1µM))
  • 10x Cas9 Nuclease Reaction Buffer

Source: E. coli BL21 (DE3) strain expressing a Cas9 gene from Streptococcus pyogenes with an N-terminal 6xHis tag and C-terminal SV40 nuclear localization signal (NLS)

Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.

Product info

Catalog Id
Size
Price
b-625-10
10 g
664.00
b-625-25
25 g
1,496.00
b-625-50
50 g
2,692.00
Description

Known in its anhydrous form as Coenzyme II, this substance is a fundamental metabolite studied in nucleotide chemistry. Nicotinamide dinucleotide phosphate (NADP) is a coenzyme carrying electrons and hydrogen in a multitude of reactions. Forms include oxidized NADP+ and reduced NADPH, a product of the pentose phosphate pathway (PPP). The molecule acts as an electron acceptor and the conjugate acid to NADP zwitterion.

NADP functions in electron transfer in the anabolic biosynthesis of lipids, cholesterol and fatty acyl chains. The substance can therefore be applied to antioxidant roles preventing oxidative damage. NADP+ and NADPH, paired together for redox reactions, participate in enzyme oxidation-reduction catalysis. NADP is further implicated in metabolism, mitochondrial conditions and apoptosis-induction.

Ferredoxin-NADP+ reductase produces NADPH in photosynthesis and is the reducing agent in the Calvin cycle, transforming CO2 into glucose for energy. NADP further reduces nitrate in the nitrogen cycle, providing the plant with ammonia. In animals, it contributes to the creation of NADPH and ADP. Reduction powers of NADPH protect these organisms from reactive oxygen species (ROS).

Product Specifications
Coenzyme II
b-NADP
NAPD+
beta-Nicotinamide adenine dinucleotide phosphate hydrate
Nicotinamide adenine dinucleotide phosphate
Triphosphopyridine nucleotide

Formula: C21H28N7O17P3 (anhydrous)

MW: 743.40 g/mol (anhydrous)

Storage/Handling: Store desiccated at 4°C. Protect from light and air.

PubChem Chemical ID: 5885

Product info

Catalog Id
Size
Price
g-040-50
50 KU
52.00
g-040-250
250 KU
202.00
g-040-1
1 MU
622.00
Description

Glucose oxidase comes from Aspergillus niger and has many applications. Glucose oxidase can be used in the determination of glucose in colorimetric assays in conjunction with peroxidase, in the determination of glucose biosensors or for use as an enzyme label.

Product Specifications
Glucose Oxidase >100 U/mg

MOLECULAR BIOLOGY GRADE

Storage/Handling: Store at -20°C.

Product info

Catalog Id
Size
Price
g-041-50
50 KU
52.00
g-041-250
250 KU
202.00
g-041-1
1 MU
622.00
Description

Glucose oxidase comes from Aspergillus niger and has many applications. Glucose oxidase can be used in the determination of glucose in colorimetric assays in conjunction with peroxidase, in the determination of glucose biosensors or for use as en enzyme label.

Product Specifications
Glucose Oxidase >225 U/mg

MOLECULAR BIOLOGY GRADE

Storage/Handling: Store at -20°C.

Product info

Catalog Id
Size
Price
p-100-100
100 mg
99.00
p-100-250
250 mg
182.00
p-100-500
500 mg
302.00
p-100-1
1 g
516.00
Description

Horseradish peroxidase (HRP) is the most commonly used antibody-conjugated enzyme. Substrates for HRP are luminol and benzidine substrates. Tetramethylbenzidine (TMB) and similar substrates are oxidized by HRP in the presence of hydrogen peroxide, or nontoxic substitutes, to yield a chinoid structure of intense blue color.

Product Specifications
Horseradish peroxidase (HRP)
Peroxidase

MOLECULAR BIOLOGY GRADE

MW: approximately 40,000 g/mol

Storage/Handling: Store desiccated at -20°C.

Product info

Catalog Id
Size
Price
p-650-40
40 μl
195.00
p-650-200
200 μl
885.00
Description

Hot Start Pfu DNA Polymerase is formulated with chemical modification which effectively neutralize 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities at room temperature, but regain the full enzyme activity upon the initial denaturation step. Hot start Pfu DNA polymerase retains the high fidelity, sensitivity and processivity of Pfu DNA polymerase, while provides reduced background by facilitating room temperature PCR assembly and preventing priming until stringent primer annealing temperatures are reached. Pfu DNA polymerase has an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

Product Includes

  • Hot Start Pfu DNA Polymerase
  • 10x PCR Buffer with Mg2+
  • 5x GC Enhancer

Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)

Concentration: 5 unit/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.

Source: E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus

Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.

Product info

Catalog Id
Size
Price
p-655-40
40 μl
216.00
p-655-200
200 μl
960.00
Description

Hot Start Pfu DNA Polymerase is formulated with chemical modification which effectively neutralize 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities at room temperature, but regain the full enzyme activity upon the initial denaturation step. Hot start Pfu DNA polymerase retains the high fidelity, sensitivity and processivity of Pfu DNA polymerase, while provides reduced background by facilitating room temperature PCR assembly and preventing priming until stringent primer annealing temperatures are reached. Pfu DNA polymerase has an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg 2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

Product Includes

  • Hot Start Pfu DNA Polymerase
  • 10x PCR Buffer with Mg2+
  • 5x GC Enhancer
  • 10mM dNTP mix

Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)

Concentration: 5 unit/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.

Source: E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus

Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.

Product info

Catalog Id
Size
Price
t-510-40
40 μl
90.00
t-510-200
200 μl
375.00
Description

Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a 5´ flap endonuclease activity. Hot Start Taq DNA Polymerase is a chemically modified enzyme that remains inactive at room temperature and requires an increase in temperature for activation. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

Product Includes

  • Hot Start Taq DNA Polymerase
  • 10x PCR Buffer with Mg2+
  • 5x GC Enhancer

Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)

Concentration: 5 units/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.

Source: E. coli strain expressing a Taq DNA Polymerase gene from Thermus aquaticus YT-1

Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.

Product info

Catalog Id
Size
Price
t-511-40
40 μl
111.00
t-511-200
200 μl
442.00
Description

Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a 5´ flap endonuclease activity. Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

Product Includes

  • Hot Start Taq DNA Polymerase
  • 10x PCR Buffer with Mg2+
  • 5x GC Enhancer
  • 10mM dNTP mix

Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)

Concentration: 5 units/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.

Source: E. coli strain expressing a Taq DNA Polymerase gene from Thermus aquaticus YT-1

Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.

Product info

Catalog Id
Size
Price
p-665-40
40 μl
172.00
p-665-200
200 μl
735.00
Description

Pfu DNA polymerase is a heat stable DNA polymerase which has 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

Product Includes

  • Pfu DNA Polymerase
  • 10x PCR Buffer with Mg2+
  • 5x GC Enhancer

Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)

Concentration: 5 unit/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.

Source: E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus

Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.

Product info

Catalog Id
Size
Price
p-690-40
40 μl
194.00
p-690-200
200 μl
810.00
Description

Pfu DNA polymerase is a heat stable DNA polymerase which has 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg 2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

Product Includes

  • Pfu DNA Polymerase
  • 10x PCR Buffer with Mg2+
  • 5x GC Enhancer
  • 10mM dNTP mix

Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)

Concentration: 5 unit/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.

Source: E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus

Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.

Product info

Catalog Id
Size
Price
p-480-sl2
2 ml
57.00
p-480-sl4
2 x 2 ml
96.00
p-480-sl10
5 x 2 ml
202.00
Description

GoldBio’s Proteinase K solution is pre-made in 20 mg/ml concentration aliquots so that you don’t have to worry about making it yourself. Now you can have the quality products that you have come to know and count on from GoldBio and combine it with the convenience of a pre-made solution.

Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.

Proteinase K is typically used at a concentration of 50-100 μg/ml. It is active with or without the presence of SDS, urea, EDTA or various metal ions, but the activity of proteinase K can be increased by adding the denaturing agents and the structure of proteinase K can be stabilized by addition of Ca 2+. Proteinase K is inactivated by heating to 95°C for 10 minutes or using an inhibitor such as PMSF, AEBSF or DFP.

Product Specifications
Proteinase K Solution – 20 mg/ml

MOLECULAR BIOLOGY GRADE

MW: 28.5 kDa

Activity: ≥30 U/mg

Unit Definition: One unit is defined as the amount of enzyme that will liberate 1.0 μmol tyrosine (Folin-positive amino acid) from casein per minute at 37°C, pH 7.5.

Storage/Handling: Store at -20°C.
Proteinase K Solution is provided in 50mM Tris-HCl (pH 8.0), 3mM CaCl2 and 50% glycerol.

Product info

Catalog Id
Size
Price
p-480-100
100 mg
74.00
p-480-500
5 x 100 mg
238.00
p-480-1
1 g
298.00
p-480-2
2 x 1 g
584.00
p-480-3
3 x 1 g
868.00
p-480-4
4 x 1 g
1,146.00
p-480-5
5 x 1 g
1,418.00
Description

Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.

Proteinase K is typically used at a concentration of 50-100 μg/ml. It is active with or without the presence of SDS, urea, EDTA or various metal ions, but the activity of proteinase K can be increased by adding the denaturing agents and the structure of proteinase K can be stabilized by addition of Ca 2+. Proteinase K is inactivated by heating to 95°C for 10 minutes or using an inhibitor such as PMSF, AEBSF or DFP.

Product Specifications
Proteinase K, RNase/DNase free

MOLECULAR BIOLOGY GRADE

MW: 28.5 kDa

Activity: ≥30 U/mg

Unit Definition: One unit is defined as the amount of enzyme that will liberate 1.0 μmol tyrosine (Folin-positive amino acid) from casein per minute at 37°C, pH 7.5.

Storage/Handling: Store at -20°C.
Reconstitute in 50mM Tris-HCl (pH 8.0), 3mM CaCl2.



Product info

Catalog Id
Size
Price
r-050-50
50 mg
58.00
r-050-100
100 mg
74.00
r-050-500
500 mg
231.00
r-050-1
1 g
350.00
r-050-5
5 g
1,544.00
r-050-10
10 g
2,998.00
Description

RNase A, isolated from bovine pancreas, degrades single-stranded RNA at the C and U residues; therefore, RNase A is pyrimidine specific. Degradation occurs by cleaving the phosphodiester bond located between the 5’-ribose of the nucleotide and the phosphate group that is connected to the 3’-ribose of the neighboring pyrimidine.

Primary applications for RNase A, which is essentially protease free, are in plasmid DNA and genomic DNA purification, RNase protection assays, and RNA sequence analysis.

Activators of this enzyme are potassium and sodium salts, while His12 and His119 can inhibit its activity.


Product Specifications
RNase A
Ribonuclease A

MOLECULAR BIOLOGY GRADE

MW: 13.7 kDa

Ideal pH: 7.5

Storage/Handling: Store at -20°C.