Agar And Plant Media

Duchefa Gelrite^TM is a naturally derived gelling polymer that can be used in a variety of applications as a solidification agent instead of agar. Produced by microbial fermentation, Gelrite^TM is a highly purified, natural anionic polysaccharide with the variations commonly associated with agar. Gelrite^TM forms rigid, brittle, agar-like gels at approximately half the use level of agar in presence of soluble salt like Mg²⁺

and Ca²⁺. Gels prepared with Gelrite™ are remarkably clear in comparison to those formed with agar. Gelrite^TM contains no contaminating matters (e.g., phenolic compounds) as found in agar that are toxic to certain sensitive organisms.

Micro Agar is a purified agar with a high gel strength and excellent properties for use in plant cell and tissue culture, as well as microbiological work. Micro Agar can be used in a minimal concentration of 5.0 g/l to obtain a solid gel.

Gel strength: >900 g/cm ²
Sulphated ash: <6%
Calcium : < 2000 ppm
Ash, acid insoluble: < 0.5%

Phyto Agar is a specially selected plant tissue culture agar with a high gel strength that is free of the extra salts of other agars which may adversely affect plant growth. Phyto Agar can be used in a minimal concentration of 5.0 g/L to obtain a solid gel.

Product Specifications
Phyto Agar

Gel Strength: 950-1050 g/cm²

Moisture: <18%

Ash Content: <3.5%

Melting Point: 85-95°C

Gelling Point: 34-36°C

GoldBio Plant agar is the preferred choice in Plant cell and Tissue culture as a general purpose agar and combines good quality with a favorable price. Agar is derived from red algae and is comprised of a mix of large polysaccharide agarose molecules and smaller, heterogeneous molecules called agaropectin.

Plant agar can be used in a minimal concentration of 5.5 g/L to to obtain a solid gel.

Product Specifications
Plant Agar;
agar-agar, Gum agar;
Bacto-Agar

Gel Strength: >1100 g/cm ²

Storage/Handling: Store at room temperature.

Preparation: Add Plant Agar to media mix at desired concentration (minimum 5.5 g/L). Autoclave media at 121°C for 20 minutes. Remove from the autoclave immediately and cool to 50°C before pouring plates.