Protein Expression & Purification

Product Specifications
1,10-Phenanthroline monohydrate

Formula: C₁₂H₈N₂ • H₂O

MW: 198.22 g/mol

Storage/Handling: Store desiccated at room temperature under inert gas.
Product is hygroscopic. 

PubChem Chemical ID: 21226

International shipments MUST be shipped by air (including Canada and Mexico). Shipments of 50 g, 100 g, and 250 g will be shipped as multiples of 25 g units to qualify for the small quantity exception.

1,2-Dipalmitoyl-rac-glycero-3-phosphocholine is a phospholipid with two palmitic acids and is the racemic mixture of Dipalmitoylphosphatidylcholine (also known as Colfosceril palmitate or DPPC). The phosphocholine head is sensitive to electric charges at the membrane surface when bound to pure phospholipids. When added to a solution containing charged ions, this sensitivity can shift the nitrogen cation away from a lack of positive charge or towards a positive charge in solution. In a research environment, it is useful in studying changes of biological membranes and liposomes. Because of its ability to form simple phospholipid bilayers, it is useful in computer modeling of bilayers in dynamic situations. Modeling can also be extended to interactions between DPPC and other biologically active molecules (like pharmaceuticals) as an analog for the same naturally occurring process. It is also useful in the formation of reconstituted HDL particles.

Product Specifications
1,2-Dipalmitoyl-rac-glycero-3-phosphocholine
2,3-di(hexadecanoyloxy)propyl 2-(trimethylazaniumyl)ethyl phosphate;
DL-1,2-Dipalmitoyl-3-phosphatidylcholine;
Dipalmitoylphosphatidylcholine (racemic)

Formula: C₄₀H₈₀NO₈P

MW: 734.04 g/mol

Storage/Handling: Store at -20^oC, and protect from light.
Forms a colorless solution when dissolved 1% in chloroform.

PubChem Chemical ID: 6138

1,2-Hexadecyliden-rac-glycero-3-phosphocholine is classified as an ether and glycerolipid. This substance can be applied as a surfactant detergent for reduction of liquid surface tension. It contains a phosphocholine group which is used in selective attachment to proteins, including C-reactive proteins responsible for initiating the phagocytotic immunologic response.

Product Specifications
1,2-Hexadecyliden-rac-glycero-3-phosphocholine
2-(N,N,N-Trimethyl)aminoethylphosphoric acid 2-heptadecyl-1,3,dioxalan-4-ylmethyl ester

Formula: C₂₄H₅₀NO₆P

MW: 479.63 g/mol

Storage/Handling: Store at -20°C. Protect from light.

PubChem Chemical ID: 6455743

1,3-Dimyristoyl-2-O-benzylglycerol is a carboxylic ester and glycerolipid containing a myristoyl group. Lipidation modification attaches the myristoyl group, which promotes weak interactions between proteins or proteins to lipids. This group is also useful in targeting membranes and signal transduction pathways. It functions as a detergent and reagent in chemical experimentation.

Product Specifications
1,3-Dimyristoyl-2-O-benzylglycerol
2-(Benzyloxy)-1,3-propanediyl ditetradecanoate
1,3-Ditetradecanoyl-2-O-benzylglycerol
Tetradecanoic acid, 2-(phenylmethoxy)-1,3-propanediyl ester

Formula: C₃₈H₆₆O₅

MW: 602.92 g/mol

Storage/Handling: Store at room temperature. 

PubChem Chemical ID: 24802114

4-Nitrophenyl phosphate, disodium salt hexahydrate is a chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of phosphatases and is used for determining alkaline phosphatase activity in human serum.

Product Specifications
4-Nitrophenyl phosphate, disodium salt hexahydrate
PNPP,
p-nitrophenyl phosphate,
para nitrophenyl phosphate,
PNP-phos · 2 Na

MOLECULAR BIOLOGY GRADE

Formula: C₆H₄NO₆PNa₂ · 6H₂O

MW: 371.15 g/mol

Storage/Handling: Store at -20°C. Protect from light.

Literature:

1. Lowry, O. H. (1957). [17] Micromethods for the assay of enzymes. 366-381.
2. Sugasawara, R. J., Prato, C. M., & Sippel, J. E. (1984). Enzyme-linked immunosorbent assay with a monoclonal antibody for detecting group A meningococcal antigens in cerebrospinal fluid. Journal of clinical microbiology, 19(2), 230-234.

Product Specifications
5-ethynyl-2’-deoxyuridine (5-EdU)

Formula: C₁₁H₁₂N₂O₅

MW: 252.22 g/mol

Storage/Handling: Store at -20°C.

PubChem Chemical ID: 472172

5-Fluoroorotic Acid (5-FOA) is widely used in the field of yeast molecular genetics. The toxicity of 5-FOA to yeast cells with a functioning URA3 gene which encodes orotidine-5′-P decarboxylase (OMP decarboxylase), makes it especially useful for the selection and identification of mutant yeast strains. 5-FOA is most often applied with Saccharomyces cerevisiae (URA3), although it has been used in other strains including Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and the bacterium E. coli (pyrF). The use of 5-FOA in the yeast 2-hybrid system allows for the construction of activation domain hybrid libraries to identify protein-protein interactions.

Product Specifications:
5-Fluoroorotic acid monohydrate (5-FOA)
5-Fluorouracil-4-carboxylic acid

MOLECULAR BIOLOGY GRADE

Formula: C₅H₃FN₂O₄ • H₂O

MW: 192.10 g/mol

White to slightly off-white crystalline powder

Storage/Handling: Store at -20°C. Protect from light.

PubChem Chemical ID: 16212749

AEBSF is an irreversible serine protease inhibitor. AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin, kallikrein and thrombin. It inhibits by acylation of the active site of the enzyme. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water and improved stability in aqueous solutions. AEBSF has been used in cell culture in concentrations of up to 0.25mM. Aqueous solutions are stable between pH 5-6; limited stability above pH 7.5.

Attention Canadian and Mexican customers: This product is a hazardous and can only ship via Air.

Product Specifications
AEBSF·HCl;
Pefabloc SC;
4-(2-Aminoethyl)-benzenesulfonylfluoride hydrochloride

MOLECULAR BIOLOGY GRADE

Formula: C₈H₁₀FNO₂S · HCl

MW: 239.69 g/mol

Storage and Handling: Store at -20°C. Protect from light.

PubChem Chemical ID: 186136

Ampicillin sodium is a member of the extended-spectrum β-lactam family and similar in structure to penicillin. It is used in the selection of antibiotic cell hybrids and has a high rate of action against many gram-negative bacterial strains. Ampicillin sodium targets non-ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. It has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus species. Resistance to ampicillin is routinely utilized as a selectable marker to confirm successful cell transformation. Ampicillin Sodium is freely soluble in water.

β-lactams inhibit the formation of peptidoglycan cross-links within bacterial cell walls by targeting penicillin-binding proteins or PBPs. Consequently, the bacterial cell wall becomes weak and cytolysis occurs. Resistance to β-lactam antibiotics occurs in the presence of cells containing plasmid encoded extended spectrum β-lactamases or ESBLs.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.

Product Specifications
Sodium Ampicillin

Formula: NaC ₁₆H₁₈N₃O₄S

MW: 371.39 g/mol

Storage/Handling: Store desiccated at -20°C. Soluble in water.

PubChem Chemical ID: 23663979

Aprotinin – from Bovine Lung

Aprotinin is a monomeric globular polypeptide derived from bovine lung tissue. It consists of a chain of 58 amino acids that fold into a stable, compact tertiary structure of the ‘small SS-rich” type, containing 3 disulfides, a twisted β-hairpin and a C-terminal α-helix.

Aprotinin inhibits several serine proteases, specifically trypsin, chymotrypsin, plasmin and kallikrein. Its action on kallikrein leads to the inhibition of the formation of factor XIIa. Binding is reversible with most aprotinin-protease complexes dissociating at pH >10 or < 3. Aprotinin is freely soluble in water (>10 mg/ml) as well as low ionic strength aqueous buffers.

Product Specifications:
Aprotinin
(Trasylol, Trypsin inhibitor (basic), Iniprol)

Formula: C₂₈₄H₄₃₂N₈₄O₇₉S₇

MW: 6511.44 g/mol

Color: White to off-white

Storage/Handling: Store at 2-8° C.

PubChem Chemical ID: 16130295

Asunaprevir is a potent inhibitor of the enzyme serine protease NS3 in hepatitis C viruses (HCV) and is currently in phase III clinical trials. This enzyme’s C terminal functions as a helicase for host DNA allowing access for viral manipulation. Asunaprevir is used in tandem with ribavirin and other direct or indirect acting antiviral compounds results in a thorough eradication of HCV.

Product Specifications
Asunaprevir

Formula: C₃₅H₄₆ClN₅O₉S

MW: 748.28 g/mol

Storage/Handling: Store at -20°C.

PubChem Chemical ID: 74891317

NOT FOR HUMAN USE!

BCIP is a white to off-white powder that is used as a substrate for visualization of alkaline phosphatase. The end product is insoluble and purple when used with nitroblue tetrazolium. This final product can be visually detected. BCIP is DMF soluble.

Product Specifications:
BCIP;
5-Bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt;
X-phos; X-phos PT

MOLECULAR BIOLOGY GRADE

Formula: C₈H₆BrClNO₄P • C₇H₉N

MW: 433.63 g/mol

Storage/Handling: Store at -20°C. Protect from light.

Benzamidine hydrochloride monohydrate is an aromatic amidine. It is used in protein extractions as a strong reversible inhibitor of serine proteases such as trypsin, thrombin, plasmin and other trypsin-like proteases. Compared to aprotinin, benzamidine is considered to be just as effective in preventing the degradation of glucogon.

Product Specifications
Benzamidine hydrochloride monohydrate

MOLECULAR BIOLOGY GRADE

Formula:C ₇H₈N₂HClH₂O

MW:174.630 g/mol

Storage/Handling:Store at -15°C, and protect from light.

Product Specifications
Bestatin

Formula: C₁₆H₂₄N₂O₄

MW: 308.37

Storage/Handling: Store at room temperature.

PubChem Chemical ID: 72172

Biotin-NHS Ester with Biotrace™ is an amine-reactive, UV-traceable biotinylation reagent that enables controllable and reproducible labeling of amine-containing biopolymers.

This advanced biotinylation reagent contains a chromophore, UV-traceable benzophenone moiety with absorption maximum of 350 nm, surrounded by two long hydrophilic PEG4 spacer arms. The hydrophilic arm enhances water solubility while reducing the tendency of some proteins to aggregate/precipitate during biotinylation. Extended PEG spacers improve conjugate yield while reducing nonspecific interactions for enhanced assay signal at higher biotin loads. After biotinylation, the conjugate’s absorbance at 280 nm and 350 nm is used to rapidly and quantitatively measure the level of biotin incorporation. Biotin-NHS Ester with Biotrace™ reagent improves labeling reproducibility and yield at higher biotin loads for maximum assay sensitivity and robustness.

The Biotin-NHS Ester with Biotrace™ Kit (Cat. # B-220-kit), provides sufficient reagents for 3 labeling reactions, each containing 50-500 μg of antibody or other lysine-containing protein in a 100 μL reaction volume. This convenient UV traceable biotin labeling reagent permits rapid quantification of incorporated biotin without the need for a HABA biotin assay.

The Biotin-NHS Ester with Biotrace™ Kit contains: Biotin-NHS Ester with Biotrace™, Anhydrous DMSO, a BupH Saline Buffer Pack and Zeba™ Spin Columns.

Formula: C₅₂H₇₆N₆O₁₇S

MW: 1089.25 g/mol

ε₃₅₀: 19,500 M^-1 cm^-1

Storage/Handling: Store at -20°C. Product shipped at ambient temperature.
Soluble in DMSO, DMF, THF or DCM.

Highlights:
– Controlled labeling – UV-traceable chromophore permits rapid, nondestructive quantification of
  incorporated biotin by means of UV-Vis spectroscopy, enabling consistency and reproducibility.
– Primary amine reactivity – reacts with primary amines (-NH2), such as the side-chain of lysines or the
  amino-termini of polypeptides.
– Extended PEG4 Spacers – impart water solubility to the biotinylated molecule, reduce or completely
  prevent aggregation of biotinylated antibodies, and minimizes steric hindrance.