Cell Lysis

Showing all 10 results

Product info

Catalog Id
Size
Price
c8001-0001
1 g
129.00
c8001-0005
5 g
250.00
c8001-0010
10 g
392.00
Description

Cellulase R-10, also called Cellulase “Onozuka” R-10, is derived from Trichoderma Viride. Cellulase R-10 is a multi-enzymatic system consisting of various enzymes, including cellulase, hemicellulase, pectinase and protease. Cellulase R-10 is typically used in association with macerozyme R-10 in the decomposition of plant cell walls to produce protoplasts.

GoldBio’s Cellulase R-10 contains >10 U/mg of cellulase activity. One unit of cellulase will release 1 µmole of glucose from carboxymethyl cellulose. Cellulase activity may be inhibited by the sugars such as glucose or cellobiose.

Product info

Catalog Id
Size
Price
c8003-0001
1 g
198.00
c8003-0005
5 g
543.00
c8003-0010
10 g
854.00
Description

Cellulase “Onozuka RS” is produced by a mutant Trichoderma viride that was derived from the parent strain for Cellulase “Onozuka -R-10”. Cellulase RS contains a very high activity of decomposing natural celluloses. This type of cellulase can be used to obtain protoplasts in a very short time and dissolves cell walls of a wider range of plants.

Product Description:
Cellulase RS, from Trichoderma Viride
Onozuka RS

Enzyme activity: >16,000 U/g

Storage/Handling: Store desiccated at 4&degC.

Product info

Catalog Id
Size
Price
d-301-50
50 mg
104.00
d-301-100
100 mg
152.00
d-301-500
500 mg
628.00
d-301-1
1 g
1,198.00
Description

DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.

Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease, which splits phosphodiester linkages preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3′ position and a phosphate group at the 5′ position. The optimum pH is 7.8.

DNase I is activated by bivalent metals and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5mM Ca2+.

Product Specifications
DNase I
Deoxyribonuclease I
DNase I, Bovine Pancreas >2000 Kunitz U/mg

Unit Definition: One Kunitz Unit will produce an A260 of 0.001 per minute at pH 5.0 at 25°C using DNA, type I or III as the substrate.

MOLECULAR BIOLOGY GRADE

Storage/Handling: Store desiccated at -20°C.
Allow product to come to room temperature before opening.

For greatest stability, it is important that DNase to be dissolved at a concentration of at least 1 mg/ml in 50% glycerol with 20mM Tris-HCl, pH 7.5, and 1mM MgCl2.

Product info

Catalog Id
Size
Price
d-300-100
100 mg
58.00
d-300-500
500 mg
172.00
d-300-1
1 g
312.00
d-300-5
5 g
1,270.00
Description

DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.

Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3′ position and phosphate group at the 5′ position. The optimum pH is 7.8.

DNase I is activated by bivalent metals, and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5mM Ca2+.

Product Specifications
DNase I
Deoxyribonuclease I
DNase I, Bovine Pancreas, >500 Kunitz U/mg

MOLECULAR BIOLOGY GRADE

Unit Definition: One Kunitz Unit will produce an A260 of 0.001 per minute at pH 5.0 at 25°C using DNA, type I or III as the substrate.

Storage/Handling: Store desiccated at -20°C.
Allow product to come to room temperature before opening.

For greatest stability, it is important that DNase to be dissolved at a concentration of at least 1 mg/ml in 50% Glycerol with 20mM Tris-HCl, pH 7.5, and 1mM MgCl2.

Product info

Catalog Id
Size
Price
e-240-100
100 mg
70.00
e-240-500
500 mg
117.00
e-240-1
1 g
210.00
e-240-5
5 g
556.00
Description

Elastase is used for tissue dissociation and cell isolation applications. Elastase has an activity of >30 units/mg and is unstable at < pH 3.5. Elastase was purified from a porcine pancreas and then purified by affinity chromatography to remove trace amounts of contaminating proteases. One unit corresponds to the amount of enzyme which liberates 1 µmol 4-nitroaniline per minute at 25°C and pH 7.8.


Product Specifications
Elastase, Porcine Pancreas

MOLECULAR BIOLOGY GRADE

Storage/Handling: Store at -20°C.

Product info

Catalog Id
Size
Price
g-211-50
50 g
32.00
g-211-100
100 g
39.00
g-211-500
500 g
68.00
g-211-1
1 kg
99.00
g-211-3
3 kg
238.00
g-211-5
5 kg
327.00
Description

Guanidine HCl is a freely soluble chaotropic agent routinely used for protein denaturation and folding.

In order to study their molecular properties and amino acid composition, proteins must be denatured from their folded state. The exact mechanism of protein unfolding by guanidine HCl is unclear. However it is speculated that the denaturant migrates into the interior of the protein and forms hydrogen bonds with atoms in the protein backbone.

Guanidine HCl is an agent frequently used to study protein properties and amino acid composition.

Product Specifications
Guanidine Hydrochloride
Guanidine HCl

Formula: CH5N3 ∙ HCl

MW: 95.53 g/mol

Storage/Handling: Store at room temperature.

PubChem Chemical ID: 5742

Product info

Catalog Id
Size
Price
l-040-1
1 g
30.00
l-040-5
5 g
66.00
l-040-10
10 g
98.00
l-040-25
25 g
172.00
l-040-100
100 g
344.00
l-040-250
250 g
792.00
Description

Lysozyme is an enzyme capable of breaking down the bacterial cell wall, and is typically used to enhance protein extractions or nucleic acid extractions. It works by hydrolyzing the 1,4-β-linkages located between the N-acetylmuramic acid and the N-acetyl-D-glucosamine residues in peptidoglycan.

Gram-positive cells have an increased susceptibility to the actions of lysozyme since gram-positive cells have a higher quantity of peptidoglycan in the cell wall. Gram-negative cells are less vulnerable, but can still be hydrolyzed in the presence of EDTA.


Product Specifications
Lysozyme, Egg White

MOLECULAR BIOLOGY GRADE

Storage/Handling: Store at -20°C. Avoid freeze/thaw cycles

Product info

Catalog Id
Size
Price
m8002-0001
1 g
129.00
m8002-0005
5 g
250.00
m8002-0010
10 g
392.00
Description

Macerozyme R-10 is a macerating enzyme from the Rhizopus sp. which is perfectly suited for the isolation of plant cells. Macerozyme R-10 is often used in combination with Cellulase “Onozuka R-10” in the isolation of plant protoplasts.

Macerozyme R-10 is a multi-component enzyme mixture containing the following characteristics:

Enzyme activity: >3,000 U/g
Pectinase : 0.5 U/mg
Cellulase: 0.1 U/mg
Hemicellulase: 0.25 U/mg
Loss on drying: <10%
solubility: 1 mg/ml 0.1M Sodium acetate buffer pH 4.5
store at 2-8°C
pH optimum: 3.5-7.0

Product info

Catalog Id
Size
Price
p8004-0001
1 g
568.00
p8004-0005
5 g
2,240.00
Description

Pectolyase Y-23 is a highly purified maceration enzyme from Aspergillus japonicus. It contains two types of pectinase such as endo-polygalacturonase and endo-pectin lyase in high activity.

Product Specifications
Pectolyase Y-23

Specific Activity: ≥1,000 maceration units per gram.

Storage/Handling: Store desiccated at 4°C.

Product info

Catalog Id
Size
Price
u-200-500
500 g
30.00
u-200-1
1 kg
50.00
Description

Urea is a nitrogenous organic compound composed of two amino groups and a carbonyl group and is commonly found as a metabolite in urine.Largely used as plant fertilizer, urea is metabolized by symbiotic urease producing soil bacteria into products readily assimilated by plants.In research, urea is commonly used in the denaturing of proteins for polyacrylamide gel electrophoresis as well as increasing the solubility of proteins in protein extraction and isolation by denaturing the insoluble inclusion bodies of recombinant proteins during production.

Product Specifications
Urea;
Carbamide

MOLECULAR BIOLOGY GRADE

Formula: CH4N2O

MW: 60.06 g/mol

Storage/Handling: Store at 2-8°C.

PubChem Chemical ID: 1176