Cell Selection & Maintenance

Showing all 14 results

Product info

Catalog Id
Size
Price
e-980-100
100 mg
88.00
e-980-250
250 mg
148.00
e-980-500
500 mg
225.00
e-980-1
1 g
338.00
Description

Product Specifications
5-ethynyl-2’-deoxyuridine (5-EdU)

Formula: C11H12N2O5

MW: 252.22 g/mol

Storage/Handling: Store at -20°C.

PubChem Chemical ID: 472172

Product info

Catalog Id
Size
Price
f-230-2-5
2.5 g
120.00
f-230-5
5 g
154.00
f-230-10
10 g
260.00
f-230-25
25 g
477.00
Description

5-Fluoroorotic Acid (5-FOA) is widely used in the field of yeast molecular genetics. The toxicity of 5-FOA to yeast cells with a functioning URA3 gene which encodes orotidine-5′-P decarboxylase (OMP decarboxylase), makes it especially useful for the selection and identification of mutant yeast strains. 5-FOA is most often applied with Saccharomyces cerevisiae (URA3), although it has been used in other strains including Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and the bacterium E. coli (pyrF). The use of 5-FOA in the yeast 2-hybrid system allows for the construction of activation domain hybrid libraries to identify protein-protein interactions.

Product Specifications:
5-Fluoroorotic acid monohydrate (5-FOA)
5-Fluorouracil-4-carboxylic acid

MOLECULAR BIOLOGY GRADE

Formula: C5H3FN2O4 • H2O

MW: 192.10 g/mol

White to slightly off-white crystalline powder

Storage/Handling: Store at -20°C. Protect from light.

PubChem Chemical ID: 16212749

Product info

Catalog Id
Size
Price
a-301-5
5 g
32.00
a-301-10
10 g
48.00
a-301-25
25 g
64.00
a-301-100
100 g
243.00
a-301-500
500 g
1,162.00
Description

Ampicillin sodium is a member of the extended-spectrum β-lactam family and similar in structure to penicillin. It is used in the selection of antibiotic cell hybrids and has a high rate of action against many gram-negative bacterial strains. Ampicillin sodium targets non-ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. It has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus species. Resistance to ampicillin is routinely utilized as a selectable marker to confirm successful cell transformation. Ampicillin Sodium is freely soluble in water.

β-lactams inhibit the formation of peptidoglycan cross-links within bacterial cell walls by targeting penicillin-binding proteins or PBPs. Consequently, the bacterial cell wall becomes weak and cytolysis occurs. Resistance to β-lactam antibiotics occurs in the presence of cells containing plasmid encoded extended spectrum β-lactamases or ESBLs.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.

Product Specifications
Sodium Ampicillin

Formula: NaC 16H18N3O4S

MW: 371.39 g/mol

Storage/Handling: Store desiccated at -20°C. Soluble in water.

PubChem Chemical ID: 23663979

Product info

Catalog Id
Size
Price
c-103-5
5 g
64.00
c-103-25
25 g
212.00
c-103-50
50 g
374.00
c-103-100
100 g
636.00
c-103-250
250 g
1,288.00
Description

Carbenicillin is a member of the penicillin β-lactam antibiotics. However, unlike most β-lactams, carbenicillin disodium is limited to primarily gram-negative bacteria including Pseudomonas aeruginosa and common enteric species. It can be used in selection protocols and is often preferred over ampicillin due to its increased stability. This allows for less contamination of unwanted bacterial colonies. In addition, carbenicillin has been used to regulate bacterial growth in plants, yielding low regeneration frequencies. It can be used in genetic transformation applications to select for AmpR transformed cells.

Penicillins are a type of β-lactam antibiotic consisting of a four-membered β-lactam ring bound to a five-membered thiazolidine ring. This two-ring system causes distortion of the β-lactam amide bond, resulting in decreased resonance stabilization and increased reactivity. β-lactams inhibit the formation of peptidoglycan cross-links within bacterial cell walls by targeting penicillin-binding proteins or PBPs. Consequently, the bacterial cell wall becomes weak and cytolysis occurs. Resistance to β-lactam antibiotics occurs in the presence of cells containing plasmid encoded extended spectrum β-lactamases or ESBLs.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.

TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS.

Product Specifications:
Carbenicillin Disodium

MOLECULAR BIOLOGY GRADE

Formula: Na2C17H16N2O6S

MW: 422.36 g/mol

Handling/Storage: Store desiccated at -20°C. Soluble in water.

PubChem Chemical ID: 20933

Product info

Catalog Id
Size
Price
c-104-1
1 g
32.00
c-104-5
5 g
57.00
c-104-25
25 g
243.00
Description

Cefotaxime is a third generation, broad-spectrum cephalosporin antibiotic effective against various gram-negative and gram-positive bacteria. However, unlike many other cephalosporins, cefotaxime is not effective against Pseudomonas aeruginosa bacteria. Cefotaxime is often used in Agrobacterium tumefaciens-mediated transformations to regulate bacterial growth. It has been shown to exhibit a synergistic effect with Vancomycin in certain conditions.

Cephalosporins are a type of β-lactam antibiotic consisting of a four-membered β-lactam ring bound to a six-membered dihydrothiazine ring. This two-ring system causes distortion of the β-lactam amide bond, resulting in decreased resonance stabilization and increased reactivity. β-lactams inhibit the formation of peptidoglycan cross-links within bacterial cell walls by targeting penicillin-binding proteins or PBPs. Consequently, the bacterial cell wall becomes weak and cytolysis occurs. Cephalosporins are less susceptible to β-lactamases than the penicillin β-lactam antibiotics.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.

TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS AT GOLD BIOTECHNOLOGY LABS

Product Specifications:
Cefotaxime, Sodium

Formula: NaC16H16N5O7S2

MW: 477.45 g/mol

Storage/Handling: Store desiccated at -20°C. Soluble in water.

PubChem Chemical ID: 10695961

Product info

Catalog Id
Size
Price
c-105-5
5 g
16.00
c-105-25
25 g
32.00
c-105-100
100 g
111.00
Description

Chloramphenicol, also known as chlornitromycin, is a broad-spectrum, bacteriostatic antibiotic derived from Streptomyces venezuelae. It is effective against several gram-positive and gram-negative bacteria and commonly used in researching protein synthesis and to select for chloramphenicol-resistant transformed cells or the bacterial CAT gene.

Chloramphenicol functions by binding the 50s ribosomal subunit and inhibiting synthesis of proteins by blocking aminoacyl-tRNA from attaching to the A site.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.

TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS AT GOLD BIOTECHNOLOGY LAB.

Product Specifications
Chloramphenicol
Chlornitromycin

MOLECULAR BIOLOGY GRADE

Formula: C11H12Cl2N2O5

MW: 323.13 g/mol

Storage/Handling: Store desiccated at -20°C. Soluble in ethanol.

PubChem Chemical ID: 5959

Product info

Catalog Id
Size
Price
g-418-1
1 g
42.00
g-418-5
5 g
194.00
g-418-10
10 g
324.00
g-418-25
25 g
477.00
Description

G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker. It can be used to select for cells transformed with an iNOS promoter construct or neomycin resistance gene.

G-418 functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation. Resistance to G-418 is conferred through the nptll gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3′-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells. G418 disulfate is freely soluble in water.

This product has been tested against both sensitive and resistant cells at Gold Biotechnology Labs.

Product Specifications:
G418;
Geneticin

Formula: C20H40N4O10 · 2H2SO4

MW: 692.71 g/mol

Storage/Handling: Store desiccated at -20°C.

PubChem Chemical ID: 16760463

Product info

Catalog Id
Size
Price
g-400-1
1 g
32.00
g-400-5
5 g
48.00
g-400-10
10 g
81.00
g-400-25
25 g
124.00
g-400-100
100 g
399.00
Description

Gentamicin is a broad-spectrum aminoglycoside antibiotic effective against gram-negative and gram-positive species. In addition, gentamicin exhibits antimicrobial properties against species resistant to tetracycline, colistin, kanamycin, and chloramphenicol.

Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.

TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS AT GOLD BIOTECHNOLOGY LABS

Product Specifications
Gentamicin Sulfate

Formula: C21H43N5O7 • H2SO4

MW: 575.67 g/mol

Storage/Handling: Store dry at -20°C.

PubChem Chemical ID (for Gentamicin; without sulfate salt): 3467

Product info

Catalog Id
Size
Price
h-270-1
1 g
96.00
h-270-2-1
2 x 1 g
178.00
h-270-2-5
2.5 g
335.00
h-270-5
5 g
398.00
h-270-5-1
5 x 1 g
430.00
h-270-10
10 g
716.00
h-270-10-1
10 x 1 g
732.00
Description

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for selection of hygromycin resistance gene transformed cells.

Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.

TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS AT GOLD BIOTECHNOLOGY LABS.

Product Specifications
Hygromycin B

Formula: C20H37N3O13

MW: 527.52 g/mol

Storage/Handling: Store desiccated at -20°C.

PubChem Chemical ID: 35766

Product info

Catalog Id
Size
Price
k-120-5
5 g
32.00
k-120-10
10 g
62.00
k-120-25
25 g
129.00
k-120-50
50 g
244.00
k-120-100
100 g
456.00
k-120-250
250 g
1,058.00
Description

Kanamycin is an aminoglycoside antibiotic isolated from Streptomyces kanamyceticus. It is effective against gram-positive and gram-negative bacteria as well as Mycoplasma species. Kanamycin is commonly used to select for bacteria that have been transformed with a kanamycin-resistant gene and plant tissues that have the NPT II (APH3) gene incorporated in them. Kanamycin is very soluble in aqueous solution and is typically used in concentrations of 50-100 µg/ml.

Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.

TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS AT GOLD BIOTECHNOLOGY LAB.

Product Specifications:
Kanamycin Monosulfate
Kanamycin A

Formula: C18H36N4O11 · H2SO4

MW: 582.58 g/mol

Storage/Handling: Store desiccated at -20°C. Soluble in water.

Activity: ~800 µg/mg

PubChem Chemical ID: 32943

Product info

Catalog Id
Size
Price
s-140-5
5 g
42.00
s-140-25
25 g
129.00
s-140-50
50 g
218.00
s-140-250
250 g
922.00
Description

Spectinomycin is an aminocyclitol antibiotic isolated from Streptomyces spectabilis. It is effective against gram-positive and gram-negative bacteria, but most commonly used against Gonococcus species. It has also been used to monitor cell fate during leaf development and as a selection marker in plants to display successful transformation of the Spcr gene.

Spectinomycin functions by binding the 30s ribosomal subunit, inhibiting tRNA translation and protein synthesis. Resistance to this antibiotic is conferred through the aadA gene and mutated rpsE gene.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.

TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS.

Product Specifications:
Spectinomycin Dihydrochloride Pentahydrate

Formula: C14H24N2O7 · 2HCl · 5H2O

MW: 495.35 g/mol

Storage/Handling: Store desiccated at -20°C.  Soluble in water.

PubChem Chemical ID: 30971

Product info

Catalog Id
Size
Price
s-150-50
50 g
32.00
s-150-100
100 g
48.00
Description

Streptomycin is an aminoglycoside antibiotic primarily effective against gram-negative bacteria and Mycobacterium species. It is commonly used in cell culture media to prevent bacterial contamination. It has also been used to precipitate nucleic acids during protein purification techniques and to study mechanisms of Streptomycin resistance.

Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited. Resistance to this antibiotic is conferred through mutations in the rpsL gene.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.

TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS.

Product Specifications

Streptomycin Sulfate

Formula: (C21H39N7O12)2 ∙ 3H2SO4

MW: 1457.4 g/mol

Storage/Handling: Store desiccated at -20°C. This product is soluble in water.

PubChem Chemical ID: 19648

Product info

Catalog Id
Size
Price
t-104-2
2 g
42.00
t-104-10
10 g
162.00
t-104-25
25 g
237.00
t-104-100
100 g
582.00
t-104-250
250 g
1,124.00
t-104-500
500 g
1,874.00
Description

Timentin™ is a 15:1 mixture of ticarcillin and clavulanate. Ticarcillin is a penicillin ß-lactam antibiotic, which is susceptible to ß-lactamase degradation. When combined with clavulanate, a ß-lactamase inhibitor, its efficacy is greatly increased. Timentin™ exhibits high activity against gram-negative bacteria and resistant Agrobacterium species.

Penicillins are a type of β-lactam antibiotic consisting of a four-membered β-lactam ring bound to a five-membered thiazolidine ring. This two-ring system causes distortion of the β-lactam amide bond, resulting in decreased resonance stabilization and increased reactivity. β-lactams inhibit the formation of peptidoglycan cross-links within bacterial cell walls by targeting penicillin-binding proteins or PBPs. Consequently, the bacterial cell wall becomes weak and cytolysis occurs. Resistance to β-lactam antibiotics occurs in the presence of cells containing plasmid encoded extended spectrum β-lactamases or ESBLs.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.

TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS AT GOLD BIOTECHNOLOGY LAB.

Product Specifications
Ticarcillin Disodium
Potassium Clavulanate

MOLECULAR BIOLOGY GRADE

Formula: C 15H14N2Na2O6S2 / C8H8KNO5

MW: 665.64 g/mol (combined)

Storage/Handling: Store desiccated at -20°C. Soluble in water.

PubChem Chemical ID (Ticarcillin): 470375
PubChem Chemical ID (Clavulanate): 23665591

Product info

Catalog Id
Size
Price
v-200-1
1 g
70.00
v-200-5
5 g
162.00
v-200-25
25 g
398.00
Description

Vancomycin hydrochloride is a member of the glycopeptide antibiotic class derived from Streptomyces orientalis. It is effective against gram-positive bacteria and used in studies of antibiotic resistance bacteria and nanoparticle transport studies. Exhibiting low toxicity to plants, vancomycin is suitable for plant selection and prevention of bacterial contamination in plant cell culture applications.

Vancomycin functions by inhibiting bacterial cell wall synthesis. It prevents transfer and addition of muramylpentapeptides that make up the peptidoglycan cell wall. It may be combined with cefotaxime or carbenicillin to display a synergistic effect against Agrobacterium species.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.

TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS AT GOLD BIOTECHNOLOGY LAB.

Product Specifications
Vancomycin Hydrochloride

Formula: C 66H75Cl2N9O24 HCl

MW: 1485.71 g/mol

Storage/Handling: Store desiccated at -20°C. Soluble in water.

Potency of greater than 1000 µg/mg

PubChem Chemical ID: 14970