Biotin

Showing all 4 results

Product info

Catalog Id
Size
Price
b-420-10
10 mg
264.00
b-420-25
25 mg
485.00
b-420-kit
1 kit
654.00
Description

Product info

Catalog Id
Size
Price
b-220-10
10 mg
178.00
b-220-25
25 mg
328.00
Description

Biotin-NHS Ester with Biotrace™ is an amine-reactive, UV-traceable biotinylation reagent that enables controllable and reproducible labeling of amine-containing biopolymers.

This advanced biotinylation reagent contains a chromophore, UV-traceable benzophenone moiety with absorption maximum of 350 nm, surrounded by two long hydrophilic PEG4 spacer arms. The hydrophilic arm enhances water solubility while reducing the tendency of some proteins to aggregate/precipitate during biotinylation. Extended PEG spacers improve conjugate yield while reducing nonspecific interactions for enhanced assay signal at higher biotin loads. After biotinylation, the conjugate’s absorbance at 280 nm and 350 nm is used to rapidly and quantitatively measure the level of biotin incorporation. Biotin-NHS Ester with Biotrace™ reagent improves labeling reproducibility and yield at higher biotin loads for maximum assay sensitivity and robustness.

The Biotin-NHS Ester with Biotrace™ Kit (Cat. # B-220-kit), provides sufficient reagents for 3 labeling reactions, each containing 50-500 μg of antibody or other lysine-containing protein in a 100 μL reaction volume. This convenient UV traceable biotin labeling reagent permits rapid quantification of incorporated biotin without the need for a HABA biotin assay.

The Biotin-NHS Ester with Biotrace™ Kit contains: Biotin-NHS Ester with Biotrace™, Anhydrous DMSO, a BupH Saline Buffer Pack and Zeba™ Spin Columns.

Formula: C52H76N6O17S

MW: 1089.25 g/mol

ε350: 19,500 M-1 cm-1

Storage/Handling: Store at -20°C. Product shipped at ambient temperature.
Soluble in DMSO, DMF, THF or DCM.

Highlights:
– Controlled labeling – UV-traceable chromophore permits rapid, nondestructive quantification of
  incorporated biotin by means of UV-Vis spectroscopy, enabling consistency and reproducibility.
– Primary amine reactivity – reacts with primary amines (-NH2), such as the side-chain of lysines or the
  amino-termini of polypeptides.
– Extended PEG4 Spacers – impart water solubility to the biotinylated molecule, reduce or completely
  prevent aggregation of biotinylated antibodies, and minimizes steric hindrance.

Product info

Catalog Id
Size
Price
b-120-100
100 mg
142.00
b-120-1000
1000 mg
292.00
Description

Biotin‐NHS ester reagent enables simple and efficient biotin labeling of amine-containing organic molecules, antibodies, proteins and any other primary amine-containing biomolecules in solution. NH2-activated biotins require an organic solvent such as DMSO or DMF to be dissolved.

Formula: C14H19N3O5S

MW: 341.38 g/mol

Storage/Handling: Store at -20°C. Product shipped at ambient temperature.
Soluble in DMSO or DMF

Highlights:
– Biotin‐NHS ester reacts rapidly with any primary amine-containing molecule to attach the biotin label via
  a stable amide bond.
– Biotin‐NHS ester is water insoluble – must be dissolved in DMSO or DMF before further dilution in
  aqueous buffers.
– Spacer arm is 13.5 angstroms.
– High purity, crystalline biotin labeling reagents can be used to create high-quality biotin-labeled
  derivatives biotinylation reagents.

Product info

Catalog Id
Size
Price
b-720-25
25 mg
142.00
b-720-100
100 mg
292.00
Description

Biotin‐PEG4‐NHS Ester is a pegylated, highly hydrophilic biotinylation reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing biomolecules.

Biotin‐PEG4‐NHS Ester reacts specifically and efficiently with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form covalent bonds. The hydrophilic polyethylene glycol (PEG) spacer arm of this reagent imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules.

Formula: C25H40N4O10S

MW: 588.67 g/mol

Net Mass Addition: 473.22 g/mol

Spacer Arm: 29Å

Storage/Handling: Store at -20°C. Product shipped at ambient temperature.
Soluble in water at 10 mg/ml.

Highlights:
– A highly hydrophilic biotinylation reagent amine reactive biotinylation reagent.
– Biotin‐PEG4‐NHS Ester increases solubility and eliminates aggregation of labeled molecules.
– Extended PEG4 spacer arm reduces steric hindrance for optimal streptavidin binding.
– High purity, solid biotin labeling reagents can be used to create high-quality biotin-labeled derivatives
   biotinylation reagents