Protein Purification

Showing all 14 results

Product info

Catalog Id
Size
Price
h-310-5
5 mL
72.00
h-310-10
10 mL
124.00
h-310-c8
His-Column 8 x 1 mL
236.00
h-310-25
25 mL
282.00
h-310-50
50 mL
470.00
h-310-c5
His-Column 5 x 5 mL
699.00
h-310-100
100 mL
840.00
h-310-500
500 mL
3,441.00
Description

Available in bulk or in prepacked columns, cobalt has a binding capacity of >25 mg/ml with approximately 60 kDa protein. Cobalt binds fewer host protein contaminants, resulting in lower background than nickel resins and no metal contamination in eluted histidine-tagged protein sample. In addition, it allows for purification of proteins under native or denaturing conditions. Cobalt Agarose works very well with Ni/Cu/Co Resin Wash Buffer and Histidine Protein Elution Buffer.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Co 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Affinity chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Storage/Handling: Store at 4°C. Do NOT freeze.

Product info

Catalog Id
Size
Price
r-203-5
5 mL
96.00
r-203-25
25 mL
422.00
r-203-100
100 mL
1,059.00
r-203-500
500 mL
4,308.00
Description

Cobalt HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Cobalt purification with the high flow rates of the HTC resin (70% higher than commercial standards).

The maximum flow rate at 15 cm bed height is >1000 cm/h and the maximum pressure is >300 kPa. The binding capacity with an approximately 30 kDA protein is 5 mg/mL.

These products are excellent for large scale His-tagged protein purifications:

  • Easy scale up and robust function
  • High chemical and physical stabilities
  • Good resolution in minimum time

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and hydrophobic interaction chromatography resins.

Storage/Handling: Store at 4°C. Do not freeze.

Product info

Catalog Id
Size
Price
g-250-5
5 mL
134.00
g-250-10
10 mL
249.00
g-250-50
50 mL
962.00
g-250-100
100 mL
1,494.00
Description

Glutathione has been covalently linked for use in affinity purification of glutathione-S-transferase (GST) and GST fusion proteins.

This product is supplied as a 75% (v/v) aqueous suspension in 20% EtOH.

Fusion proteins expressed from pGEX vectors contain a Glutathione S-transferase (GST) moiety and can therefore be purified to near homogeneity by affinity chromatography on glutathione as a substrate in inactivate toxic small molecules via formation of mercapturic acid. Because the affinity of GST for its substrate is in the submillimolar range, immobilization of glutathione on an agarose matrix makes a highly efficient affinity chromatography resin.

This product provides a one-step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Bound GST –fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione.

Storage/Handling: Store at 4°C. Do not freeze.

Product info

Catalog Id
Size
Price
i-901-25
25 mL
108.00
i-901-50
50 mL
160.00
i-901-100
100 mL
246.00
i-901-250
250 mL
570.00
Description

Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.

Recrystallized Imidazole from GoldBio is specially purified Imidazole specifically designed for Histidine tagged protein purification. We purify the Imidazole at Gold Biotechnology’s labs and go through multiple purification steps including ultrafiltration and recrystallization to obtain the most purified Imidazole on the market. The final Imidazole product is tested in our labs for elution of tagged protein from Ni columns.

Product Specifications:
Sterile filtered and recrystallized 3M Imidazole;
Glyoxaline;
1,3-diazacyclopenta-2,4-diene

Fomula: C3H4N2

MW= 68.08 g/mol.
(20.42 g/100 mL)

Storage/Handling: Store at 4°C

PubChem Chemical ID: 795

Product info

Catalog Id
Size
Price
i-902-25
25 g
92.00
i-902-50
50 g
154.00
i-902-100
100 g
248.00
i-902-500
500 g
758.00
Description

Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins. Imidazole is also commonly used as a buffer for enzymatic reactions and has a buffering pH range of 6.2 – 7.8.

Recrystallized Imidazole from GoldBio is specially purified Imidazole designed for histidine tagged protein purification. We purify the Imidazole at Gold Biotechnology’s labs and go through multiple purification steps including ultrafiltration and recrystallization to obtain the most purified Imidazole on the market. The final Imidazole product is tested in our labs for elution of tagged protein from Ni columns.

Product Specifications:
Sterile filtered and recrystallized Imidazole;
Glyoxaline;
1,3-diazacyclopenta-2,4-diene

Molecular Biology Grade
Protease Free

Formula: C3H4N2

MW: 68.08 g/mol

Ships in 25 g units

PubChem Chemical ID: 795

Product info

Catalog Id
Size
Price
n-201-25
25 g
32.00
n-201-50
50 g
44.00
n-201-100
100 g
78.00
n-201-250
250 g
150.00
Description

NDSB-201 is a protein specific additive that can be used to help solubilize and stabilize proteins at non-denaturing concentrations while preventing protein aggregation.

NDSB-201 is a member of a group of nondetergent sulfobetaines. These zwitterionic compounds can reduce aggregation and aid in refolding and renaturing of proteins found in bacterial expression systems and in inclusion bodies. Unlike detergents such as CHAPS, NDSBs are nondenaturing, even at higher concentrations and have been shown to increase the yield efficiency of membrane and nuclear proteins, among others.

NDSBs are highly soluble in water and aqueous solutions and do not significantly affect the pH of buffered solutions and can easily be removed by dialysis. These compounds are not considered detergents due to their hydrophilic sulfobetaine group and short hydrophobic groups which prevents them from aggregating and forming micelles.

Product Specifications:
NDSB-201 (Non-detergent sulfobetaine);
3-(1-Pyridino)-1-propane sulfonate

Formula: C8H11NO3S

MW: 201.24 g/mol

Storage/Handling: Store desiccated at room temperature.

PubChem Chemical ID: 84929

Product info

Catalog Id
Size
Price
h-320-5
5 mL
72.00
h-320-10
10 mL
124.00
h-320-c8
Column: 8x1 mL
236.00
h-320-25
25 mL
282.00
h-320-50
50 mL
470.00
h-320-c5
Column: 5x5 mL
699.00
h-320-100
100 mL
840.00
h-320-500
500 mL
3,441.00
Description

Nickel Agarose Resin is available in bulk or in prepacked columns. It has a binding capacity of >25 mg/ml with approximately 60 kDa protein. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Storage/Handling: Store at 4°C. Do NOT freeze.

Note: Some discoloration of the Nickel IDA resin has been observed in reaction with low levels of reducing agents such as DTT. However, the resin remains fully functional even at concentrations as high as 5mM DTT.

Product info

Catalog Id
Size
Price
h-322-ca1
Cartridge: 1x5mL
264.00
h-322-ca5
Cartridge: 5x5mL
1,032.00
Description

Product info

Catalog Id
Size
Price
r-202-5
5 mL
96.00
r-202-25
25 mL
422.00
r-202-100
100 mL
1,059.00
r-202-500
500 mL
4,308.00
r-202-1000
1000 mL
7,524.00
Description

Nickel HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Nickel purification with the high flow rates of the HTC resin (70% higher than commercial standards).

The Maximum flow rate at 15 cm bed height is > 1000 cm/h and the maximum pressure is greater than 300 kPa. The binding capacity with an approximately 30 kDA protein is 6 mg/mL.

These products are excellent for large scale His-tagged protein purifications:
-Easy scale up and robust function
-High chemical and physical stabilities
-Good resolution in minimum time

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates, with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and Hydrophobic interaction chromatography resins.

Storage/Handling: Store at 4°C. Do not freeze.

Note: Some discoloration of the Nickel IDA resin has been observed in reaction with low levels of reducing agents such as DTT. However, the resin remains fully functional even at concentrations as high as 5mM DTT.

Product info

Catalog Id
Size
Price
h-350-5
5 ml
93.00
h-350-10
10 ml
150.00
h-350-25
25 ml
327.00
h-350-50
50 ml
566.00
h-350-100
100 ml
1,068.00
Description

Nickel-NTA Agarose Resin has a binding capacity of ~50 mg/ml. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

NTA cross-linked Agarose resin consists of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent, covalently coupled to cross-linked agarose beads, providing a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Storage/Handling: Store at 4°C. Do not freeze.

Note: GoldBio recommends using Nickel NTA resins in the presence of elevated levels of reducing agents in order to ensure optimal purification of your target protein. Nickel IDA resins may show some discoloration in reaction with low levels of reducing agents but are still functional at low levels of reducing agents (≤5mM DTT).

Product info

Catalog Id
Size
Price
h-351-2
2 ml
150.00
h-351-5
5 ml
338.00
h-351-10
10 ml
612.00
Description

Nickel NTA Magnetic Agarose Beads are a rapid and easy small scale purification of histidine-tagged proteins. This resin consists of magnetic agarose derivatized with Nitrilotriacetic (NTA) and provides good properties working in native or denaturing conditions. Magnetic agarose beads provide a convenient and quick method for purification without the need for pipetting or centrifugation. Washing, binding and elution steps will require a magnetic device.

NTA cross-linked resins consist of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent which provides a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Nickel NTA Magnetic Beads are supplied as a 5% suspension of magnetic beads in 20% ethanol (each 1 ml of suspension contains 50 μl of magnetic beads).

Loading Capacity (μmol me2+/ml gel): >20 µmol/ ml gel

Binding Capacity: >75 mg His-tagged protein/ ml gel

Storage/Handling: Store at 4°C. Do not freeze.

Note: GoldBio recommends using Nickel NTA resins in the presence of elevated levels of reducing agents in order to ensure optimal purification of your target protein. Nickel IDA resins may show some discoloration in reaction with low levels of reducing agents but are still functional at low levels of reducing agents (≤5mM DTT).

Product info

Catalog Id
Size
Price
p-400-5
5 ml
243.00
p-400-25
25 ml
952.00
p-400-50
50 ml
1,754.00
p-400-100
100 ml
3,004.00
Description

Protein A agarose resin consists of rProtein A covalently bound to cross-linked agarose beads. It provides a very stable bond that can greatly minimize leakage of the Protein A allowing for reuse of the affinity resin in several purification steps. Protein A Agarose resin is available in bulk from 5-100 ml quantities.

Protein A resin is good for the purification of a wide range of Immunoglobulins of different mammalian species and also certain IgG subclasses that have no other affinity (see additional information).

Protein A is a cell wall component of Staphylococcus aureus. It consists of a single polypeptide chain shaped as a cylinder, which contains five antibody–binding domains. These high affinity regions are specifically bonded to Fc region of the immunoglobulins (specially IgGs of different species).

Protein A is temperature stable and it retains its native conformation even in the presence of denaturing agents.

Storage/Handling: Store at 4°C. Do not freeze.

Product info

Catalog Id
Size
Price
p-430-1
1 ml
190.00
p-430-5
5 ml
542.00
p-430-25
25 ml
1,881.00
Description

Protein G HTC Agarose 4% Resin

Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains of native Protein G have been removed to ensure the maximum specific IgG binding capacity.

GoldBio offers rProtein G resins to isolate and purify classes, subclasses and fragments of immunoglobulins from cell culture media and biological fluids. Rapid purification and high yield of purified immunoglobulin are obtainable by this method.

Protein G is covalently bound to highly cross-linked agarose beads, providing a very stable bond that can greatly minimize leakage of the Protein G and allowing for reuse of the affinity resin in several purification steps. This resin works well in High Flow conditions.

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly cross-linked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity chromatography and base media support for producing IEX (Ion Exchange) and hydrophobic interaction chromatography resins.

Storage/Handling: Store at 4°C. Do NOT freeze.

Product info

Catalog Id
Size
Price
s-105-1
1 ml
93.00
s-105-5
5 ml
352.00
s-105-10
10 ml
626.00
Description

Streptavidin Agarose Resin is a high affinity biotin-binding chromatography resin with streptavidin covalently bound to 6% crosslinked agarose beads and a biotin binding capacity of >120 nmol/ml of gel. Immobilized Streptavidin resin is suitable for gravity flow columns, spin columns, and FPLC methods. Immobilized Streptavidin can be used for affinity chromatography purifications by separating biotinylated proteins from nonbiotinylated proteins and for affinity purification of antigens when used with biotinylated antibodies.

Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces avidinii. Streptavidin can bind to four moles of biotin per mole of protein and it shows extremely high affinity, approximately 10-15 kDa. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point of 6.5 to avidin’s 10, far closer to that at which most useful biological interactions occur. As a result, Streptavidin frequently exhibits much lower nonspecific binding than avidin. It has an acidic pH of 5.5 and a mass of 75 kDa.

Superior coupling chemistry: The methods used to prepare this resin are superior to all other immobilization technologies, providing higher binding capacity, lower nonspecific binding, and less leaching.
Available in multiple sizes: The product is available in 1, 2, 5, & 10 ml bulk quantities.

Applications:
• Purification of membrane antigens in conjunction with biotinylated monoclonal antibodies
• Cell-surface labeling with biotinylation reagents, followed by precipitation with immobilized streptavidin
• Purification of cell-surface glycoproteins using biotinylated Concanavalin A
• Recovery of single-stranded biotinylated DNA for dideoxy sequencing

Storage/Handling: Store at 4°C. Do not freeze.