Bis-acrylamide is the most frequently used crosslinking agent for protein separation applications in polyacrylamide gels. Bis-acrylamide powder is freely soluble in water (20 mg/mL) and 2% bis-acrylamide solution is a standard solution used in most electrophoresis applications.
Bis-acrylamide is used to create crosslinks between acrylamide to generate polyacrylamide gel in electrophoresis gels. The ratio of bis-acrylamide to acrylamide manipulates the porous characteristics of the polyacrylamide gel.
MOLECULAR BIOLOGY GRADE
MW: 154.17 g/mol
Storage/Handling: Store at 2-8 °C.
Product is air and light sensitive.
PubChem Chemical ID: 8041
Protocol for the preparation of an SDS-PAGE gel and electrophoresis of protein samples.