Phosphoenolpyruvic acid, trisodium salt heptahydrate is used as a reagent for the determination of glycerol. It is also a substrate for phosphoenolpyruvate carboxylase, which is an enzyme that catalyzes the addition of bicarbonate to phosphoenolpyruvate (PEP).
In bacteria, PEP acts as a source of energy for the phosphotransferase system (PTS). This mechanism involves the enzymatic extraction of a phosphate group from phosphoenolpyruvate. The phosphate group is then carried between several proteins until it reaches the sugar transporter known as EIIC.
Furthermore, PEP is used in research involving traditional cell-free protein reactions. These reactions require enough stable energy for protein synthesis. Since PEP holds the highest-energy phosphate bond among living organisms, PEP is commonly used for these reactions to produce ATP necessary for translation.
MOLECULAR BIOLOGY GRADE
Color/Purity:White to off-white crystalline powder
Storage/Handling:Store at -15°C.
P-180-10, P-180-2.5, P-180-25, P-180-250, P-180-5, P-180-50