Thiazolyl blue tetrazolium bromide (often called MTT) is a common dye used in Cell Proliferation assays or Cell Growth assays. MTT is converted to water-insoluble, dark blue MTT-formazan by mitochondrial dehydrogenases of living cells. The intensity of the color can then be measured colorimetrically at a wavelength of 570 nm.
MW: 414.32 g/mol
Handling/Storage: Store dark at 4°C, protect from light/ air, keep dry.
Soluble in Water or PBS up to 5 mg/ml, and in Ethanol at 20 mg/ml.
PubChem Chemical ID: 64965
T-030-1, T-030-10, T-030-2.5, T-030-5