Validamycin is a fungicidal, aminoglycoside antibiotic known to inhibit trehalases in plants, insects, and fungi as well as enhance trehalose accumulation in transgenic plants. It is effective against Pellicularia sasakii and Rhizoctonia solani in plants, but only decreases their virulence instead of exhibiting a fungicidal effect. In addition, validamycin is used to control sheath-blight disease in rice and is most effective against soil-borne pathogens. It is used to study trehalase activity and the synthesis of trehalose.
Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.
Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.
MOLECULAR BIOLOGY GRADE
MW: 497.49 g/mol
Storage/Handling: Store desiccated at -20°C. Soluble in water.
PubChem Chemical ID 443629